Polyinosinic–polycytidylic acid potassium salt
SIGMA/P9582 - with buffer salts, TLR ligand tested
Synonym: Poly (I:C); Poly(I) • Poly(C)
CAS Number: 31852-29-6
MDL Number: MFCD00131983
Product Type: Chemical
Polyinosinic–Polycytidylic acid potassium salt (Poly I:C) (Cat. No. P9582) induces proinflammatory cytokines through TLR3-TRIF complex.Cells (macrophage cells, WT and macrophages deficient in TRIF adaptor protein, TRIF KO) were plated (8×104/well) and incubated for 4 hours. Poly I:C was then added at the indicated concentrations. Twenty hours following Poly I:C addition supernatants were tested for TNFα concentration using quantitative sandwich ELISA. The assay was performed in triplicates and mean values ±SD are shown. In collaboration with Gabriel Nussbaum, Faculty of Dental Medicine, The Hebrew University of Jerusalem.
Polyinosinic–polycytidylic acid sodium salt unit structure.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9582-50MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9582-5MG
| assay | ≥99% (less than 1% free nucleotides, TLC) |
| composition | Poly(I) • Poly(C), 10% (balance buffer salts as sodium chloride and sodium phosphate) |
| form | lyophilized powder |
| Quality Level | 300  |
| storage condition | desiccated |
| storage temp. | −20°C |
| Application: | TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidyli |
| Biochem/physiol Actions: | Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine. |
| Other Notes: | Double-stranded homopolymer. |
| Packaging: | 5, 50 mg in glass bottle |
| Packaging: | Package size based on polynucleotide content |
| Preparation Note: | The product requires ionic strength to maintain the double-strand structure. Reconstitution at ~10 mg/mL of water yields a polynucleotide in physiological phosphate buffered solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (less than 1% free nucleotides, TLC) |
| Storage Temp. | −20°C |
| UNSPSC | 41106305 |