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o-Phenylenediamine

SIGMA/P9029 - Peroxidase substrate, ≥98.0%, powder

Synonym: 1,2-Diaminobenzene; 1,2-Phenylenediamine; OPD

CAS Number: 95-54-5
Empirical Formula (Hill Notation): C6H8N2
Molecular Weight: 108.14
EC Number: 202-430-6
MDL Number: MFCD00007721
Linear Formula: C6H8N2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-P9029-50G 50 g
 $239.00
1/EA		 Add To Favorites
45-P9029-100G 100 g
 $434.00
1/EA		 Add To Favorites
o-Phenylenediamine (OPD) is a chromogenic substrate suitable for use in ELISA procedures which utilize horseradish peroxidase conjugates. This substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3 M H2SO4, and read at 492 nm.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9029-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9029-50G

 

assay ≥98.0%
bp 256-258 °C
color white to off-white
fluorescence λex 337 nm; λem 417 nm (Pyruvic acid derivative)
form powder
InChI 1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2
InChI key GEYOCULIXLDCMW-UHFFFAOYSA-N
mp 98-102 °C
Quality Level 200 
SMILES string Nc1ccccc1N
solubility ethanol: 50 mg/mL, clear, colorless to faintly yellow
storage temp. 2-8°C
vapor density 3.7 (vs air)
vapor pressure 0.01 mmHg ( 25 °C)
Application: o-Phenylenediamine has been used in the microtiter?based assay for hyaluronan quantification. It has also been used in enzyme linked immunosorbent assay (ELISA) for color development.
Disclaimer: May darken in storage.
General description: o-Phenylenediamine (OPD) Free Base is a peroxidase substrate suitable for use in enzyme linked immunosorbent assay (ELISA) procedures. The substrate produces a soluble product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
Packaging: 50, 100 g in glass bottle
Purity ≥98.0%
bp 256-258 °C
mp 98-102 °C
Storage Temp. 2-8°C
UNSPSC 12352204

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