Protease Inhibitor Cocktail
SIGMA/P8340 - for use with mammalian cell and tissue extracts, DMSO solution
Synonym: Protease Inhibitor Mix
MDL Number: MFCD00677817
Product Type: Chemical
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| biological source | bovine lung |
| form | DMSO solution |
| Quality Level | 300  |
| solubility | water: soluble |
| storage temp. | −20°C |
| Application: | Ideal for use as a buffer component during sonication, lysis buffer, and RIPA buffer Provides protection against endogenous enzyme activity in cell and tissue extracts |
| Biochem/physiol Actions: | Inhibits serine, cysteine, and acid proteases, and aminopeptidases. |
| Biochem/physiol Actions: | This mixture contains individual components, including AEBSF at 104 mM, Aprotinin at 80 μM, Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases. |
| Disclaimer: | This cocktail should be stored at -20 °C. |
| Features and Benefits: | Broad-spectrum inhibition: inhibits multiple classes of proteases and aminopeptidases Stable storage: supplied as a solution in DMSO, allowing for long-term storage at -20°C Consistent performance: ready-to-use solution |
| General description: | Protease Inhibitor Cocktail for mammalian cell and tissue extracts, in DMSO solution. It contains six individual components for inhibiting serine, cysteine, and acid proteases, and aminopeptidases. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | AEBSF Aprotinin Bestatin E-64 Leupeptin Pepstatin A |
| Packaging: | 1, 5 mL in serum bottle |
| Preparation Note: | One mL of this cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g of bovine liver or 10 mL of lysate from CHO cells. This product is supplied as a solution in DMSO. |
| WGK Germany | WGK 2 |
| Flash Point(F) | 188.6 °F - closed cup |
| Flash Point(C) | 87 °C - closed cup |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |