Pertussis toxin from Bordetella pertussis
SIGMA/P7208 - lyophilized powder
Synonym: Histamine-
CAS Number: 70323-44-3
MDL Number: MFCD00131841
Product Type: Chemical
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| form | lyophilized powder |
| InChI key | WOPKHAQDUMDJIY-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | O(C)C(=O)CCCCCCCCCCCCCCCC |
| storage temp. | 2-8°C |
| Application: | Inactive toxin form released from B. pertussis. |
| Application: | Pertussis toxin from Bordetella pertussis has been used: as a blocker of the Gαi subunit of the chemokine (CXCR4) receptor to measure the intracellular Ca2+ in breast cancer cell lines, to characterize the Gi signaling involved in ADP-induced tissue factor (TF) and P-selectin exposure, to induce autoimmune vasculitis in a rat model |
| Application: | The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al. |
| Biochem/physiol Actions: | Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. |
| Biochem/physiol Actions: | Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels. |
| Features and Benefits: | This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm . |
| Features and Benefits: | This compound is featured on the G Proteins (Heterotrimeric) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here . |
| General description: | Pertussis toxin (PT) is a multi-subunit complex toxin that possesses one active subunit(A) and five binding subunits (B5). PT enters the mammalian cells via endocytosis by attaching itself to the glycosylated molecules on the surface of these cells. |
| Physical form: | Lyophilized powder containing sodium chloride and sodium phosphate buffer salts |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |