Anti-Protein Disulfide Isomerase (DL-11) antibody produced in rabbit
SIGMA/P7122 - affinity isolated antibody, buffered aqueous solution
Synonym: Prolyl 4-hydroxylase subunit β (P4HB); Anti-Erp58; Anti-PDI
MDL Number: MFCD01094568
Product Type: Chemical
Immunofluorescence
HeLa cells were fixed and permeabilized with 3% paraformaldehyde followed by 0.5% Triton-X-100. Fixed cells were stained with 5 μg/mL Anti-PDI (Cat. No. P7122). The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
HeLa cells were fixed and permeabilized with 3% paraformaldehyde followed by 0.5% Triton-X-100. Fixed cells were stained with 5 μg/mL Anti-PDI (Cat. No. P7122). The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Enhanced Validation-By Independent Antibodies HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton. Fixed cells were stained with 5 μg/mL Anti-Protein Disulfide Isomerase (DL-11) antibody produced in Rabbit (Cat. No. P7122) (A) and developed using Goat Anti-Rabbit IgG, Cy3 conjugate, and 5 μg/mL Anti-Protein Disulfide Isomerase (MD-12) antibody produced in Rabbit (Cat. No. P7372) (B). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate antibody. Results Two Anti-Protein Disulfide Isomerase antibodies,P7122 (A) And P7372 (B) target different regions of Protein Disulfide Isomerase show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 57 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1-2 μg using RIPA lysate (250-500 μg) of rat NRK cells |
| indirect immunofluorescence: 2-5 μg/mL using human HeLa cells | |
| western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of mouse NIH3T3 cells | |
| UniProt accession no. | P07237  |
| Application: | Anti-Protein Disulfide Isomerase (DL-11) antibody produced in rabbit has been used in: • immunofluorescence • immunoprecipitation • immunoblotting |
| Biochem/physiol Actions: | Prolyl 4-hydroxylase subunit β (P4HB) acts as a molecular chaperone in the endoplasmic reticulum of cells and also as an oxidoreductase. It associates with steroid hormones and modulates their actions, concentrations and storage. P4HB accelerates the formation of disulphide bonds in proteins and hence aids in their folding. |
| Biochem/physiol Actions: | Protein Disulfide Isomerase serves as a molecular chaperone, that can suppress protein aggregation. PDI has functions as an essential component of two protein complexes: the heterotetramer collagen prolyl 4-hydroxylase and the heterodimer microsomal triglyceride transfer protein. PDI participates in the hydroxylation of prolines in procollagen during collagen synthesis and in the transfer of neutral lipid onto nascent lipoprotein particles. PDI has calcium-dependent transglutaminase activity, which catalyzes the formation of isopeptide bonds. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Prolyl 4-hydroxylase subunit β (P4HB) is a redox-regulated thiol-containing protein. The gene encoding this protein is localized on human chromosome 17q25.3. |
| General description: | Protein Disulfide Isomerase is an abundant multifunctional, soluble enzyme (E.C. 5.3.4.1). PDI is expressed in cellular localizations such as the cell surface, cytosol and nucleus. PDI consists of four tandem domains, two of which contain a catalytic site for S-S bond formation. One domain is the main site of noncovalent interaction with other peptides or proteins. PDI has an N-terminal ER signal and C-terminal ER retention KDEL signal sequences. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 498-508 of human protein disulfide isomerase. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |