HIS-Select® Nickel Affinity Gel
SIGMA/P6611 - (1:1 suspension in a 20% ethanol solution)
Synonym: Ni-NTA resin; nickel charged agarose
Product Type: Chemical
Figure 1. One-Step Purification
| capacity | >15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein) |
| color | faint blue to very dark blue |
| concentration | 1.5-2.4 mL/mL (suspension in packed gel) |
| conjugate | magnetic beads |
| feature | hydrophilic |
| form | (1:1 suspension in a 20% ethanol solution) |
| matrix | 6% Beaded Agarose |
| packaging | pkg of 1 mL |
| pkg of 100 mL | |
| pkg of 25 mL | |
| pkg of 5 mL | |
| pkg of 500 mL | |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | protein purification: suitable |
| transition temp | flash point 32 °C (closed cup) |
| Application: | HIS-Select® Nickel Affinity Gel has been used in the purification of recombinant proteins like EF-hand calcium-binding protein (S100A14), LIM homeobox transcription factor 1 alpha protein, sigma-1 receptor as well as harpin, stable protein 1 (SP1), and BCR-ABL fusion protein. |
| Features and Benefits: | • High selectivity for higher purity. • Unique non-charged hydrophilic linkage reduces non-specific binding. • Binding capacity for histidine-tagged protein is greater than 15 mg/mL. • Binding under denaturing or non-denaturing conditions. • One-step purification. • Minimizes unwanted ionic interactions. • Minimal nickel leaching. • Bead size: 45-165 μm. |
| General description: | HIS-Select® Nickel Affinity Gel is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography. |
| Legal Information: | HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | It is also available with the EZview™ technology (Product Code E3528). |
| Physical form: | 1:1 suspension in a 20% ethanol solution |
| Preparation Note: | HIS-Select Nickel Affinity Gel is stable for at least one year when stored properly. The HIS-Select Nickel Affinity Gel should be cleaned after each use and an antimicrobial agent such as 20% ethanol should be added to the storage buffer. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P233 - P240 - P241 - P370 + P378 - P403 + P235 |
| Risk Statements | 10 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| WGK Germany | WGK 3 |
| Flash Point(F) | 89.6 °F - closed cup |
| Flash Point(C) | 32 °C - closed cup |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |