Papain from papaya latex
SIGMA/P4762 - lyophilized powder, ≥10 units/mg protein
Synonym: Papainase
CAS Number: 9001-73-4
EC Number: 232-627-2
MDL Number: MFCD00131791
Product Type: Chemical
Papain is a cysteine protease of the peptidase C1 family. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Papain is used routinely for the preparation of Fab fragments from IgG. IgM may also be digested with papain resulting in high yields of homogeneous Fab preparations.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P4762-25MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P4762-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P4762-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P4762-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P4762-50MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P4762-500MG
| application(s) | diagnostic assay manufacturing |
| biological source | papaya (latex) |
| form | lyophilized powder |
| mol wt | 23.4 kDa |
| Quality Level | 300  |
| specific activity | ≥10 units/mg protein |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| single cell analysis: suitable |
| Application: | Papain from papaya latex has been used:• to digest carotid artery smooth muscle cells • in dissecting solutions • to produce Fab fragments of antibodies • for cell dissociation since it has been shown to be more effective and less damaging with certain tissues • in the enzyme cocktail for dissociating human fetal retinal tissue • to digest chondrocytes/scaffold cultures for total DNA quantification for cell proliferation • as a supplement in neurobasal medium to produce retinal cell suspensions Suitable for use in preparation of single cell suspension for sequencing. |
| Application: | Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. |
| Biochem/physiol Actions: | A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine. pH optimum 6.0-7.0 Also hydrolyzes esters and amides. |
| Biochem/physiol Actions: | Papain with wide specificity, cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. Papain is considered to digest protein substrates more broadly than the pancreatic proteases. Papain is commonly used in cell isolation procedures as it is highly efficient and less harmful than other proteases on certain tissues. The use of papain is known to yield more viable smooth muscle cells, retaining the cell′s sensitivity to stimulants. Papain papaya latex has antifungal activity against C. albicans and is a cysteine protease. • The pH optimum is 6.0-7.0 • Papain hydrolyzes esters and amides |
| General description: | Papain is a cysteine protease, found in Carica papaya. It is composed of a single polypeptide chain containing three disulfide bridges and a sulfhydryl group. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginin |
| Other Notes: | View more information on papain at www.sigma-aldrich.com/enz. |
| Packaging: | 1, 5 g in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Packaging: | 50, 100, 500 mg in glass bottle |
| Packaging: | Package size based on protein content |
| Physical form: | Lyophilized powder containing sodium acetate |
| Preparation Note: | 2× Crystallized |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.22.2 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |