Pectinase from Aspergillus niger
SIGMA/P4716 - BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)
Synonym: Poly-(1,4-α-D-galacturonide) glycanohydrolase; Polygalacturonase solution from Aspergillus niger
CAS Number: 9032-75-1
EC Number: 232-885-6
MDL Number: MFCD00131809
Product Type: Chemical
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| biological source | Aspergillus niger |
| form | aqueous glycerol solution |
| greener alternative category |  , Enabling |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| product line | BioReagent |
| Quality Level | 200 |
| specific activity | ≥5 units/mg protein (Lowry) |
| storage temp. | 2-8°C |
| sustainability | Greener Alternative Product |
| technique(s) | cell culture | plant: suitable |
| Application: | Petctinase from Aspergillus niger has been used: • for protoplasts liberation • in the protoplast enzyme mixture for enzyme digestion • to conduct partial saccharification of sugars • to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications |
| Application: | Used in plant protoplast preparation to digest cell wall prior to organelle isolation. |
| Biochem/physiol Actions: | Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research . |
| Other Notes: | One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 25000, 100000 units in glass bottle |
| Physical form: | Solution in 40% glycerol |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥5 units/mg protein (Lowry) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.15 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |