Protease from Bacillus licheniformis
SIGMA/P3910 - lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A
Synonym: Proteinase from Bacillus licheniformis; Subtilisin A; Proteinase from Bacillus licheniformis; Subtilisin A
CAS Number: 9014-01-1
EC Number: 232-752-2
MDL Number: MFCD00132092
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3910-500MG
| application(s) | food and beverages |
| form | lyophilized powder |
| mol wt | 27 kDa |
| quality | for use in Total Dietary Fiber Assay, TDF-100A |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Application: | This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A). |
| Biochem/physiol Actions: | Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions. |
| Biochem/physiol Actions: | Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphat |
| General description: | Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa. |
| Other Notes: | One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C. |
| Packaging: | 500 mg in poly bottle |
| Symbol |     GHS05,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 - H334 - H335 - H410 |
| Precautionary statements | P273 - P280 - P301 + P312 + P330 - P302 + P352 - P305 + P351 + P338 + P310 |
| Hazard Codes | Xn |
| Risk Statements | 22-37/38-41-42-50 |
| Safety Statements | 22-24-26-36/37/39-61 |
| RIDADR | UN 3082 9 / PGIII |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.21.62 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |