Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4
SIGMA/P3563
Synonym: 1X PBS + 0.05% TWEEN® 20; 1X PBS + 0.05% TWEEN® 20; PBS-T; PBST
Product Type: Chemical
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| application(s) | cell analysis diagnostic assay manufacturing |
| form | powder |
| Quality Level | 200  |
| solubility | water: soluble, clear, colorless (1 pouch dissolved in 1L) |
| Application: | Phosphate buffered saline has been used in: • western blotting • enzyme-linked immunosorbent assay (ELISA) • telomere-FISH (Tel-FISH) and telomere/immunostaining-F • cell isolation |
| General description: | Phosphate buffered saline with TWEEN® 20, or PBS-T, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN 20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBS-T buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Packaging: | Foil pouches |
| Preparation Note: | Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41105319 |