Phalloidin–Tetramethylrhodamine B isothiocyanate
SIGMA/P1951 - sequence from Amanita phalloides(synthetic: peptide sequence)
Synonym: Phalloidin-TRITC
Empirical Formula (Hill Notation): C60H70N12O13S2
Molecular Weight: 1231.40
MDL Number: MFCD00278840
Linear Formula: C60H70N12O13S2
Product Type: Chemical
Immunofluorescence Phalloidin–Tetramethylrhodamine B isothiocyanate: Cat. No. P1951: Septins are small GTPases that localize in a filamentous pattern in cells. Treatment of cultured Human podocytes with cytochalasin D disrupts the filamentous organization of the actin cytoskeleton, as visualized by phalloidin staining (red). In cytochalasin D-treated podocytes, the normally linear septin 7 filaments (green) curl and form ring-like structures, indicating that septin 7 localization depends on an intact actin cytoskeleton. The nucleus is visualized with DAPI (blue). Submitted by A. Wasik, University of Helsinki.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P1951-.1MG
| biological source | sequence from Amanita phalloides (synthetic: peptide sequence) |
| fluorescence | λex 540-545 nm; λem 570-573 nm |
| form | solid |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | Fluorescent phallotoxin which may be used to identify filamentous actin. Phalloidin-Tetramethylrho • In Immunofluorescence for staining Filamentous actin (F-actin) • To stain cells during immunocytochemical and cytochemical analysis • To label actin microfilaments for fluorescence microscopy |
| Biochem/physiol Actions: | Phalloidin interacts with polymeric actin, and not oligomeric or monomeric forms. This interaction leads to highly stabilized actin filaments, which resist depolymerization and disassembly. In rats, this toxin causes death due to liver hemorrhage, and cells show abnormal actin clustering. The affinity of phalloidin to actin is not significantly altered after derivatizing florescent labelled phalloidin compounds. These compounds can be used to study actin structure and organization within eukaryotic cells. |
| Biochem/physiol Actions: | Toxin that binds polymeric F actin, stabilizing it and interfering with the function of actin-rich structures. |
| General description: | Phalloidin is a phallotoxin produced by death cap mushroom Amanita phalloides. It is a cyclic peptide, which interacts with actin, and this was first identified in phalloidin-poisoned rats. It is a heptapeptide, cyclic in nature, with a crosslink between tryptophan at position 6 and cysteine at position 3. The side chain of amino acid 7 (γ-δ-dihydroxyleucine) in phalloidin, is accessible to modifications, through which florescent labelled phalloidin compounds can be produced. |
| Other Notes: | May contain mixed isomers |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 + H310 + H330 |
| Precautionary statements | P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310 |
| Hazard Codes | T+ |
| Risk Statements | 26/27/28 |
| Safety Statements | 22-28-36/37-45 |
| RIDADR | UN 3462 6.1 / PGII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352116 |