Phosphorylase a from rabbit muscle
SIGMA/P1261 - lyophilized powder, 20-30 units/mg protein
Synonym: 1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase
CAS Number: 9032-10-4
EC Number: 232-878-8
MDL Number: MFCD00167428
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| biological source | rabbit muscle |
| composition | Protein, 30-60% E |
| foreign activity | AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1% |
| phosphoglucomutase ≤0.25% | |
| phosphorylase b ≤30% | |
| form | lyophilized powder |
| purified by | 2× crystallization |
| Quality Level | 200  |
| specific activity | 20-30 units/mg protein |
| storage temp. | −20°C |
| Application: | Phosphorylase a from rabbit muscle has been used to mix with glycogen for the preparation of limit dextrin. It has also been used to probe the array in oligosaccharide microarray analysis of phosphorylase activity. |
| Application: | Phosphorylase from rabbit muscle has been used in a study to assess the molecular mechanisms of oleanolic acid. It has also been used in a study to describe the conversion of phosphorylase B to A, through a conversion enzyme in the presence of 32P-ATP. |
| Biochem/physiol Actions: | Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase. |
| Biochem/physiol Actions: | Phosphorylase A can be inhibited by these compounds: Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls. |
| Biochem/physiol Actions: | Phosphorylase participates in glycogenolysis. Deficiency of phosphorylase leads to several glycogen storage diseases with hepatomegaly. |
| General description: | Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P). |
| Other Notes: | One unit will form 1.0 μmole of α- |
| Packaging: | 10, 25 mg in poly bottle |
| Physical form: | Lyophilized powder containing β-glycerophosphate and EDTA |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 20-30 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.4.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |