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pT7-FLAG™-2 Expression Vector

SIGMA/P1243

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-P1243-10UG 10 µg
 $416.00
1/EA		 Add To Favorites

 

grade Molecular Biology
Quality Level 200 
recombinant expressed in E. coli
shipped in dry ice
storage temp. −20°C
General description: pT7-FLAG-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.

The C-terminal FLAG fusion protein may be detected using Monoclonal ANTI-FLAG?M2, Catalog Number
F3165, and purified using the ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.

Vector Maps and Sequences 
Legal Information: FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information: pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC
Other Notes: • pT7-FLAG-2 Expression Vector, 10 μg (P6867) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
• pT7-FLAG-2-BAP Control Vector 1 μg ( P7117) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
RIDADR NONH for all modes of transport
Storage Temp. −20°C
UNSPSC 41106610

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