PARP-1 human
SIGMA/P0996 - recombinant, expressed in E. coli
Synonym: NAD+ ADP-
MDL Number: MFCD01324157
Product Type: Chemical
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| form | solution |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P09874  |
| Application: | PARP-1 human has been used in the GST-pull down assay for immunoprecipitation of Glutathione S-transferase (GST) or GST- cyclic GMP- AMP synthase (cGAS) (GST-cGAS). It has also been used in in vitro ribosylation assay. |
| Application: | PARP-1 is a nuclear enzyme that synthesizes ADP-ribose polymers from NAD+, specifically binds Zn2+ and DNA, and recognizes single-strand breaks in DNA. PARP1 has been used in a study to assess racial and tissue-specific cancer risk associated with polymorphism in the PARP1 gene. It has also been used in a study to investigate inhibitors of PARP-1 for potential cancer treatments. |
| Biochem/physiol Actions: | PARP-1 is inactivated by cleavage into a 24kDA and 89kDA fragment by activated caspase-3 or caspase-7. This results in the decreased ability to repair DNA damage and an increase in apoptosis. |
| Biochem/physiol Actions: | PARP-1, a nuclear enzyme that synthesizes ADP-ribose polymers from NAD, specifically binds Zn2+ and DNA, and recognizes single-strand breaks in DNA. It is involved in base excision repair, both short-patch and long-patch, rejoining DNA strand breaks and plays a role in p53 expression and activation. A high level of basal neuronal DNA damage and PARP activity has been reported in rat brain tissue. PARP-1 was shown to be required for HIV-1 integration into DNA. If PARP-1 is deficient there is no productive HIV-1 infection. |
| Biochem/physiol Actions: | Poly(ADP-ribose) polymerase 1 (PARP1) is essential for diverse functions like DNA damage detection and repair, chromatin modification, cell differentiation, transcription and apoptotic cell death. It also plays a major role in spermatogenesis. Polymorphism in the gene PARP1 is associated with oligospermia and causes male infertility. |
| General description: | Poly(ADP-ribose) polymerase 1 (PARP-1) is associated with the inflammation response during atherosclerosis. |
| General description: | Poly(ADP-ribose) polymerase 1 (PARP1) belongs to the PARP family which is highly conserved during evolution. PARP1 gene is localized at 1q42.12 in the human chromosome. |
| Legal Information: | Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow |
| Other Notes: | One unit will incorporate 10 femptomole of poly(ADP-ribose) from NAD into 5 μg of immobilized histone proteins in 30 minutes at 22 °C in a 96 well plate. |
| Packaging: | 1000 units in glass bottle |
| Physical form: | Supplied as a solution in 20 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM DTT, 0.1% Triton™-X 100, 50 % glycerol, and 0.1 mg/ml BSA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.4.2.30 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |