Oncostatin M human
SIGMA/O9635 - BioReagent, ≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: OSM
CAS Number: 106956-32-5
EC Number: 200-835-2
MDL Number: MFCD00166731
Product Type: Chemical
Bioassay for Oncostatin M The biological activity of Oncostatin M (Cat. No. O9635) is measured in a cell proliferation assay using the human erythroleukemic cell line, TF-1. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.
| assay | ≥97% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | ~22 kDa |
| packaging | pkg of 10 μg |
| potency | ≤1.000 ng/mL ED50 |
| product line | BioReagent |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | soluble 0.100 mL, clear, colorless (Solvent-PBS + 0.1% BSA) |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P13725  |
| Analysis Note: | The proliferative activity of human oncostatin M is tested in culture by using a human erythroleukemic cell line, TF-1. |
| Application: | Human oncostatin-M has been used to stimulate bovine full-depth cartilage explants. It has also been used to culture embryonic stem cells in standard minimum essential medium-α. |
| Biochem/physiol Actions: | Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. It can exert both stimulatory and inhibitory effects on cell proliferation. OSM stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi’s sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release [e.g. interleukin-6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte-colony-stimul |
| Biochem/physiol Actions: | Oncostatin M (OSM), LIF, G-CSF, IL-6, and CNTF are structurally related members of the same cytokine family sharing similarities in their primary amino acid sequences, predicted secondary structure, and receptor components. OSM is a growth-regulating cytokine, affecting a number of tumor and normal cells. This material was first identified by its ability to inhibit the growth of A375 melanoma cells and other human tumor cells, but not inhibit the growth of normal human fibroblasts. It acts synergistically with TGF β1 to inhibit the proliferation of tumor cells like A375 melanoma cells. It induces an increase in LDL receptor expression and LDL uptake by hepatoma cells. OSM activates synovial fibroblast-like cells to produce urokinase type plasminogen activator. OSM is secreted by macrophages and activated T lymphocytes. |
| General description: | Oncostatin M (OSM) is produced by activated T cells, monocytes and Kaposi’s sarcoma cells. OSM shares several structural and functional characteristics with leukemia inhibitory factor (LIF), interleukin-6 (IL-6), and ciliary neurotrophic factor (CNTF). |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 10 mM acetic acid, containing 50 μg of bovine serum albumin per 1 μg of cytokine. |
| Hazard Codes | F,Xn |
| Risk Statements | 11-20/21/22-36 |
| Safety Statements | 16-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |