Anti-Oct 3/4 antibody produced in rabbit
SIGMA/O8389 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-OTF3/OTF4; Anti-Octamer-binding transcription factor 3/4; Anti-POU class 5 homeobox 1; Anti-POU5F1
Product Type: Chemical
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| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:100-1:250 |
| western blot: 1:500-1:1,000 | |
| UniProt accession no. | Q01860  |
| Application: | Anti-Oct 3/4 antibody produced in rabbit has been used in immunocytochemistry and immunofluorescence staining. |
| Biochem/physiol Actions: | Oct 3/4 maintains developmental potency and governs distinct fates of embryonic stem cells. A moderate increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct 3/4 stimulates loss of pluripotency and dedifferentiation to trophectoderm. POU domain class 5 transcription factor 1 (POU5F1) serves as a master regulator of pluripotency in embryonic stem cells. It is one of the reprogramming factors, that is highly essential for generating induced pluripotent stem cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | POU domain class 5 transcription factor 1 (POU5F1) is also termed as OCT4. It is a transcription factor. OCT4 codes for three isoforms, such as OCT4A, OCT4B and OCT4B1. These isoforms are produced by alternative splicing. The OCT4 gene consists of 7 exons. This gene is located on human chromosome 6. OCT4A and OCT4B are located in the nucleus and cytoplasm respectively. |
| Immunogen: | synthetic peptide corresponding to an internal sequence of human Oct 3/4. This sequence is 84% identical in mouse and 94% identical in rat. |
| Physical form: | solution in phosphate buffered saline, containing 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |