Anti-Osteopontin antibody produced in rabbit
SIGMA/O7264 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Bone Sialoprotein 1; Anti-Early T Lymphocyte Activation Protein 1 (ETA1); Anti-Secreted Phosphoprotein 1; Anti-Uropontin
MDL Number: MFCD02263865
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human kidney were stained with 5 μg/mL Anti-Osteopontin antibody produced in Rabbit (Cat. No. O7264), followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of 293T cells were separated on SDS-PAGE and probed with Anti-Osteopontin antibody produced in Rabbit (Cat. No. O7264). The antibody was developed using 1:20,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545). Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using human kidney |
| western blot: 0.5-1 μg/mL using whole extract of human embryonal kidney 293T cells and a chemiluminescent detection reagent | |
| UniProt accession no. | P10451  |
| Application: | Anti-Osteopontin antibody produced in rabbit has been used in immunohistochemistry and immunoblotting. |
| Biochem/physiol Actions: | Osteopenia (OPN), functions like chemokine and cytokine. It is implicated in a number of physiologic and pathologic events including angiogenesis, apoptosis, inflammation, wound healing and tumor metastasis. Osteopontin expression is upregulated in cancer of breast, prostate, ovarian, lung, brain tumor and hepatocellular carcinoma. |
| Biochem/physiol Actions: | Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Osteopontin (OPN), also termed as bone sialoprotein 1, urinary stone protein, early t lymphocyte activation protein 1 (ETA1), and uropontin, is a phosphorylated acidic arginine-glycine-aspartat |
| General description: | Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 249-267 of human osteopontin, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |