Anti-O-GlcNAc Transferase (TI-14) antibody produced in rabbit
SIGMA/O6014 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-O-linked N-Acetylglucosamine Transferase; Anti-OGT
MDL Number: MFCD07370422
Product Type: Chemical
Immunoblotting
Extracts of HeLa cell lysate (Lane 1), HeLa nuclear (Lane 2) and recombinant OGT expressed from baculovirus system (Lane 3) were separated on SDS-PAGE and blotted with Rabbit Anti-OGLcNAc transferase (OGT) (Cat. No. O6014) and (Cat. No. O6264 and O6139). The antibodies were developed with Goat Anti-Rabbit IgG, Peroxidase conjugate and chemiluminescent substrate.
Extracts of HeLa cell lysate (Lane 1), HeLa nuclear (Lane 2) and recombinant OGT expressed from baculovirus system (Lane 3) were separated on SDS-PAGE and blotted with Rabbit Anti-OGLcNAc transferase (OGT) (Cat. No. O6014) and (Cat. No. O6264 and O6139). The antibodies were developed with Goat Anti-Rabbit IgG, Peroxidase conjugate and chemiluminescent substrate.
Immunofluorescence Cytoplasm, nuclear and mitochondria localization of OGLcNAc transferase (OGT) in human lung carcinoma (A549) cells stained by Rabbit Anti-OGLcNAc Transferase (OGT) (TI-14), IgG fraction of antibody (Cat. No. O6014). A. Working dilution of 1:100 B. Working dilution of 1:50 C. Anti-Cytochrome C (Cat. No. C5723)
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 110 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:50-1:100 using A549 human lung carcinoma cells fixed with paraformaldehyde/triton |
| western blot: 1:1,000-1:2,000 using HeLa cell nuclear extracts | |
| UniProt accession no. | O15294  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Biochem/physiol Actions: | O-linked β-N-acetyl glycosamine (O-GlcNAc) is involved in repressing transcription. O-GlcNAc transferase (OGT) interacts with a histone deacetylase complex by binding to the co repressor Sin3A. This interaction leads to the repression of transcription, after transcription factors and RNA polymerase II are modified by addition of O-GlcNAc. O-GlcNAc modification reversibly inhibits proteosomal function in an ubiquitin-independent fashion. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | O-GlcNAc transferase (OGT) catalyzes the addition of an N-acetylglucosamine residue to the amino acids serine or threonine. The enzyme is an homotrimer consisting of three subunits of 110 kDa each, with multiple tetratricopeptide (TPR) repeats. |
| Immunogen: | synthetic peptide corresponding to amino acids 1024-1037 of human O-GlcNAc transferase, conjugated to KLH via an N-terminal added cysteine residue. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |