GenElute™ Bacterial Genomic DNA Kits
SIGMA/NA2110 - sufficient for 70 purifications
Synonym: Bacterial Genomic DNA; bacterial DNA prep; bacterial DNA purification kit; bacterial gDNA extraction kit; bacterial gDNA isolation kit; bacterial gDNA purification kit; bacterial genomic DNA extraction kit; bacterial genomic DNA isolation kit; bacterial genomic DNA purification kit; Gen Elute
Product Type: Chemical
Comparison of Gram– bacteria genomic DNA isolation kits.
Agarose gel analysis of genomic DNA isolated from the indicated Gram– bacteria prepared using the GenElute™ Bacterial Genomic DNA Kit versus kits from other suppliers. Equal proportions of DNA were resolved on a 1%, 1X TBE agarose gel. The lambda Hind III ladder (Cat. No. D9780) was used as a size standard (M).
Agarose gel analysis of genomic DNA isolated from the indicated Gram– bacteria prepared using the GenElute™ Bacterial Genomic DNA Kit versus kits from other suppliers. Equal proportions of DNA were resolved on a 1%, 1X TBE agarose gel. The lambda Hind III ladder (Cat. No. D9780) was used as a size standard (M).
Comparison of Gram+ bacteria genomic DNA isolation kits.
Agarose gel analysis of genomic DNA isolated from the indicated Gram+ bacteria prepared using the GenElute™ Bacterial Genomic DNA Kit versus kits from other suppliers. Equal proportions of eluted product were resolved on a 1%, 1X TBE agarose gel. The lambda Hind III ladder (Cat. No. D9780) was used as a size standard (M).
Agarose gel analysis of genomic DNA isolated from the indicated Gram+ bacteria prepared using the GenElute™ Bacterial Genomic DNA Kit versus kits from other suppliers. Equal proportions of eluted product were resolved on a 1%, 1X TBE agarose gel. The lambda Hind III ladder (Cat. No. D9780) was used as a size standard (M).
PFGE of Bacterial gDNA isolated with GenElute™ Bacterial gDNA Kit
Purified genomic DNA was isolated from various bacterial species using the GenElute™ Bacterial Genomic DNA kit. A 1 μg aliquot of DNA from each respective bacterial sample was resolved on a 1% agarose gel in 0.5X TBE at 150 volts for 16 hours using a BioRad CHEF DRII system. The initial pulse time was 2 seconds, the final pulse time was 13 seconds, the start ratio was 1.0, pump speed was set at 70, and PFGE was carried out at 4 °C. M represents the 0.1–200 kb Pulse marker (Cat. No. D2291).
Lanes
1. E. coli
2. P. fluorescens
3. B. subtilis
Purified genomic DNA was isolated from various bacterial species using the GenElute™ Bacterial Genomic DNA kit. A 1 μg aliquot of DNA from each respective bacterial sample was resolved on a 1% agarose gel in 0.5X TBE at 150 volts for 16 hours using a BioRad CHEF DRII system. The initial pulse time was 2 seconds, the final pulse time was 13 seconds, the start ratio was 1.0, pump speed was set at 70, and PFGE was carried out at 4 °C. M represents the 0.1–200 kb Pulse marker (Cat. No. D2291).
Lanes
1. E. coli
2. P. fluorescens
3. B. subtilis
| Quality Level | 200  |
| storage temp. | 15-25°C |
| technique(s) | DNA purification: suitable |
| usage | sufficient for 70 purifications |
| Application: | The purified bacterial genomic DNA is ready for downstream applications such as: • restriction endonuclease digestions • PCR • Southern blots • cloning |
| Biochem/physiol Actions: | The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length. |
| Features and Benefits: | • Starting material: Up to 1.5 mL of culture • Expected yield: Up to 20 μg • Elution volume: 400 μl • Time required: 70 - 120 min • A260/A280 ratio: 1.6 - 1.9 • No phenol, chloroform, or ethanol precipitation required |
| Features and Benefits: | • Typical DNA yields of 15 μg - 20 μg • Protocols provided for Gram + and Gram - bacteria • High quality genomic DNA in less than 2 hours • Purified DNA has an A260/A280 ratio between 1.6 and 1.9 |
| General description: | Click here to view the kit protocol |
| General description: | The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions. The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | For additional information, please see www.sigma-aldrich.com/gen. |
| Packaging: | Components included:• Qty in kit:3 Part number: P2308 Component description: Proteinase K • Qty in kit:3 Part number: T5449 Component description: Collection Tubes, 2 mL • Qty in kit:1 Part number: C2112 Component description: Column Preparation Solution • Qty in kit:1 Part number: R6148 Component description: RNase A solution • Qty in kit:1 Part number: L7539 Component description: Gram-Positive Lysis Solution • Qty in kit:1 Part number: CP9471 Component description: GenElute Miniprep Binding Columns • Qty in kit:1 Part number: B6803 Component description: Elution Solution (10 mM Tris-HCl, 0.5 mM EDTA, pH 9.0) • Qty in kit:1 Part number: B8803 Component description: Lysis Solution C • Qty in kit:1 Part number: W0263 Component description: Wash Solution 1 • Qty in kit:1 Part number: B6678 Component description: Lysis Solution T • Qty in kit:1 Part number: B6553 Component description: Wash Solution Concentrate |
| Symbol |      GHS02,GHS05,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H226 - H290 - H302 - H315 - H318 - H334 - H336 - H410 |
| Precautionary statements | P210 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 10-22-36/37/38-42-52/53-67 |
| Safety Statements | 23-26-45-61 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | 77.0 °F - closed cup |
| Flash Point(C) | 25 °C - closed cup |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |
| Components | Wash Solution 1; Column Preparation Solution; Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein; RNase A solution |