GenElute™ PCR Clean-Up Kit
SIGMA/NA1020 - sufficient for 70 purifications
Synonym: Gen Elute; PCR Purification
Product Type: Chemical
Sequence was resolved on an ABI 3100 from a purified, 645 bp corn leaf PCR product.
The PCR product was purified with the GenElute™ PCR Clean-Up Kit. The DNA extraction and PCR were performed using Sigma′s Extract-N-Amp™ Plant PCR Kit. The sequence was obtained by using ABI BigDye® terminator chemistry and the same primers as the original PCR.
The PCR product was purified with the GenElute™ PCR Clean-Up Kit. The DNA extraction and PCR were performed using Sigma′s Extract-N-Amp™ Plant PCR Kit. The sequence was obtained by using ABI BigDye® terminator chemistry and the same primers as the original PCR.
Comparison of PCR product recovery and primer removal.
Three separate PCR products were purified with the GenElute™ PCR Clean-Up Kit and a comparable kit from Supplier Q. Products were 143 bp from corn leaf, 375 bp from pBR322, 2 kb from human blood. Samples were analyzed on a 20% TBE acrylamide gel and visualized by staining with SYBR® Green II
Lanes
1. Unpurified Reaction
2. GenElute™ PCR Clean-Up Kit
3. Supplier Q
Three separate PCR products were purified with the GenElute™ PCR Clean-Up Kit and a comparable kit from Supplier Q. Products were 143 bp from corn leaf, 375 bp from pBR322, 2 kb from human blood. Samples were analyzed on a 20% TBE acrylamide gel and visualized by staining with SYBR® Green II
Lanes
1. Unpurified Reaction
2. GenElute™ PCR Clean-Up Kit
3. Supplier Q
Sequence was resolved on an ABI 3100 from a purified, 645 bp corn leaf PCR product.
The PCR product was purified with the GenElute™ PCR Clean-Up Kit. The DNA extraction and PCR were performed using Sigma′s Extract-N-Amp™ Plant PCR Kit. The sequence was obtained by using ABI BigDye™ terminator chemistry and the same primers as the original PCR.
The PCR product was purified with the GenElute™ PCR Clean-Up Kit. The DNA extraction and PCR were performed using Sigma′s Extract-N-Amp™ Plant PCR Kit. The sequence was obtained by using ABI BigDye™ terminator chemistry and the same primers as the original PCR.
| Quality Level | 200  |
| storage temp. | 15-25°C |
| technique(s) | DNA purification: suitable |
| usage | sufficient for 70 purifications |
| Application: | GenElute™ PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction. |
| Application: | Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis. |
| Biochem/physiol Actions: | The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed). |
| Features and Benefits: | • Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes • Recovers up to 95% of PCR products between 100 bp and 10 kb • Removes over 99% of primers and other components • Eliminates the need to remove mineral oil by organic extraction • 40% more purification preps supplied than market leader |
| Features and Benefits: | • Recovers up to 95% of PCR products between 100 bp and 10 kb • Purifies up to 100 μL or 10 μg of PCR amplified DNA in 8 minutes • Removes over 99% of primers and other components |
| General description: | Click here to view the kit protocol |
| General description: | The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform. DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform. |
| Packaging: | Components included:• Qty in kit:2 Part number: T5449 Component description: Collection Tubes, 2 mL • Qty in kit:1 Part number: B7556 Component description: Binding Solution • Qty in kit:1 Part number: W3637 Component description: Wash Solution Concentrate • Qty in kit:1 Part number: E7777 Component description: Elution Solution (10 mM Tris-HCl, pH 8.5) • Qty in kit:1 Part number: G6415 Component description: GenElute™ plasmid mini spin column • Qty in kit:1 Part number: C2112 Component description: Column Preparation Solution |
| Symbol |   GHS05,GHS07 |
| Signal word | Warning |
| Hazard statements | H290 - H302 - H315 - H319 - H336 |
| Precautionary statements | P301 + P312 + P330 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/38-67 |
| Safety Statements | 26 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 15-25°C |
| UNSPSC | 41106300 |