GenElute™ HP Plasmid Maxiprep Kit
SIGMA/NA0300 - sufficient for 10 purifications
Synonym: GenElute™ HP Plasmid Maxiprep Kit
Product Type: Chemical
Solutions are drawn through the column by vacuum instead of centrifugation, increasing speed and decreasing hands on time.
GenElute High Performance (HP) Plasmid Kits
Comparison of time required per isolation using different Maxiprep plasmid isolation systems. Plasmid DNA was isolated following each manufacturers recommended protocol. Each system was tested multiple times and the average preparation time is shown here.
Comparison of plasmid DNA yield using different Maxiprep plasmid isolation systems. Plasmid DNA was isolated following each manufacturers recommended protocol. All samples were done in duplicate.
Comparison of transfection efficiencies of plasmids prepared with different plasmid isolation methods. Data shows an average of 3 replicates for each named isolation method. All transfections were in CHO-K1 cells. The OD measurements were taken at 420 nm recording the units of β-gal/plate. The cells were grown to 60–70% confluency and transfected using 3 μg of plasmid pSPORT-CMV/15 μg of Escort IV. β-Gal activity was determined 54 hours post-transfection.
Purified plasmid DNA is suitable for restriction enzyme digestions. Restriction digestion of plasmid DNA isolated using the GenElute™ HP Plasmid Maxiprep Kit and a comparable kit from Supplier Q. The plasmid DNA (800 ng) was digested with 10 units of Hind III at 37 °C for 4 hours. Each plasmid sample represents 100 ng of undigested DNA (left) and digested DNA (right) loaded in each pair of lanes. Samples were loaded on a 1% TBE agarose gel. The marker (M) used was a 1 kb DNA Ladder (Cat. No. D0428).
Electropherogram revealing >700 bases of sequence from pCMV-SPORT-βgal purified from GenElute™ HP Plasmid Midiprep Kit. Cycle Sequencing was performed using 500 ng template, a T7 sequencing primer and ABI BigDye® terminator chemistry. Sequencing reactions were resolved on an ABI Prism® 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE Plus acrylamide at 2.4 kV, 48 °C for 7 hours.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| storage temp. | 15-25°C |
| sustainability | Greener Alternative Product |
| technique(s) | DNA purification: suitable |
| usage | sufficient for 10 purifications |
| Application: | GenElute™ HP Plasmid Maxiprep Kit has been used in plasmid DNA extraction of SF91 or MIEV vector, apolipoprotein A-V mutant constructs, lentiviral vector pLenti6/R4R2/V5-DEST,metastasis-associated protein 1 (MTA1) and adipokinetic hormone receptor |
| Features and Benefits: | • From harvested bacterial culture to pure plasmid DNA in 30 minutes or less • Up to 1.2 mg of high-copy plasmid DNA • Flexibility of a vacuum or spin format • Contains fewer plastic components than other high speed kits, reducing the amount of waste • No phenol/chloroform extraction or alcohol precipitation required |
| General description: | Click here to view the kit protocol |
| General description: | The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used. An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water. The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | For additional information, please see www.sigma-aldrich.com/gen. |
| Packaging: | Components included:• Qty in kit:2 Part number: C4353 Component description: Collection Tubes, 50mL capacity • Qty in kit:1 Part number: L1912 Component description: Lysis Solution • Qty in kit:1 Part number: W4639 Component description: Wash Solution 2 • Qty in kit:1 Part number: R6148 Component description: RNase A solution • Qty in kit:1 Part number: N1285 Component description: Neutralization Solution • Qty in kit:1 Part number: G4917 Component description: GenElute™ HP Maxiprep Binding Column • Qty in kit:1 Part number: R1149 Component description: Resuspension Solution • Qty in kit:1 Part number: C2112 Component description: Column Preparation Solution • Qty in kit:1 Part number: W0263 Component description: Wash Solution 1 • Qty in kit:1 Part number: G9042 Component description: GenElute™ HP Maxiprep Filter Syringe • Qty in kit:1 Part number: B4683 Component description: Binding Solution • Qty in kit:1 Part number: E7777 Component description: Elution Solution (10 mM Tris-HCl, pH 8.5) |
| Symbol |     GHS02,GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H290 - H302 - H314 - H334 - H336 |
| Precautionary statements | P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 10-22-36/38-67 |
| Safety Statements | 26 |
| RIDADR | UN 3316 9 |
| Flash Point(F) | 77.0 °F - closed cup |
| Flash Point(C) | 25 °C - closed cup |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |