GenElute™ HP Plasmid Miniprep Kit
SIGMA/NA0160 - sufficient for 350 preparations
Synonym: GenElute™ HP Plasmid Kit; HP miniprep kit; fast miniprep kit; fast plasmid isolation kit; plasmid extraction kit; plasmid isolation kit; plasmid purification kit; ultrafast plasmid isolation kit; Gen Elute
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| storage temp. | 15-25°C |
| sustainability | Greener Alternative Product |
| technique(s) | DNA extraction: suitable |
| usage | sufficient for 350 preparations |
| Application: | The recovered plasmid DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transcription, and sequencing. |
| Features and Benefits: | • Purified plasmid DNA in less than 30 minutes • Up to 25 μg of high-copy plasmid DNA • Flexibility of a vacuum or spin format • No phenol/chloroform extraction or alcohol precipitation required |
| General description: | Click here to view the kit protocol |
| General description: | An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Contaminants are then removed by a vacuum or spin wash steps. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer. The GenElute™ HP Plasmid Miniprep Kit offers an ultrafast and efficient solution for plasmid preparation from E. coli cultures. This kit combines silica-based membrane technology and the convenience of spin or vacuum format,up to 25 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimum volume of culture to use depend on the plasmid and the culture medium. |
| General description: | The GenElute HP Plasmid Miniprep Kit offers an ultrafast and efficient solution for plasmid preparation from E. coli cultures. This kit combines silica-based membrane technology and the convenience of spin or vacuum format. An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Contaminants are then removed by a vacuum or spin wash steps. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer. The recovered plasmid DNA is ready for immediate use in applications such as restriction digest, cloning, PCR, transfection, and sequencing. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | Components included:• Qty in kit:10 Part number: T5449 Component description: Collection Tubes, 2 mL • Qty in kit:5 Part number: G8667 Component description: GenElute HP Miniprep Binding Columns • Qty in kit:1 Part number: N5158 Component description: Neutralization/Binding Buffer • Qty in kit:1 Part number: E7777 Component description: Elution Solution (10 mM Tris-HCl, pH 8.5) • Qty in kit:1 Part number: R1149 Component description: Resuspension Solution • Qty in kit:1 Part number: W0263 Component description: Wash Solution 1 • Qty in kit:1 Part number: R6148 Component description: RNase A solution • Qty in kit:1 Part number: C2112 Component description: Column Preparation Solution • Qty in kit:1 Part number: W4639 Component description: Wash Solution 2 • Qty in kit:1 Part number: L1912 Component description: Lysis Solution |
| Symbol |    GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H290 - H302 - H314 - H334 - H336 |
| Precautionary statements | P234 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/38-67 |
| Safety Statements | 26 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |