4-Nitrophenyl N-acetyl-β-D-glucosaminide

SIGMA/N9376 - ≥99% (TLC)

Synonym: 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside

CAS Number: 3459-18-5
Empirical Formula (Hill Notation): C₁₄H₁₈N₂O₈
Molecular Weight: 342.30
EC Number: 222-398-7
MDL Number: MFCD00063696
Linear Formula: C₁₄H₁₈N₂O₈
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-N9376-100MG	100 mg	$66.80 1/EA	Add To Favorites
45-N9376-250MG	250 mg	$128.00 1/EA	Add To Favorites
45-N9376-500MG	500 mg	$237.00 1/EA	Add To Favorites
45-N9376-1G	1 g	$281.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N9376-100MG

Properties
Descriptions
Safety Info.
Basic Data

assay	≥99% (TLC)
form	powder
impurities	≤0.05% Free p-nitrophenol
InChI	1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1
InChI key	OMRLTNCLYHKQCK-DHGKCCLASA-N
Quality Level	300
SMILES string	CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc(cc2)[N+]([O-])=O
solubility	water: 5 mg/mL, clear, colorless to very faintly yellow
storage temp.	−20°C

Application:	N-acetyl-β-D-glucosaminidase (NAG) has been used: • as a substrate to assay N-acetyl-β-D-glucosaminidase (NAG) • as a substrate to study chitinase ChiB1 activity • to assess degranulation using rat basophilic leukemia (RBL)-2H3 cells
Biochem/physiol Actions:	4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.
General description:	Chromogenic substrate for N-acetyl-glucosaminidase.
Packaging:	1 g in poly bottle
Packaging:	100, 250, 500 mg in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99% (TLC)
Storage Temp.	−20°C
UNSPSC	12352204

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