Neocarzinostatin from Streptomyces carzinostaticus
SIGMA/N9162 - ≥90% (SDS-PAGE), ~0.5 mg/mL
Synonym: Holoneocarzinostatin; NCS; NSC-69856; Zinostatin
CAS Number: 9014-02-2
MDL Number: MFCD01778130
Product Type: Chemical
Immunofluorescence U2-OS cells were treated with increasing concentrations of Neocarzinostatin (NCS) (Cat. No. N9162) (A,B—20 ng, C,D—50 ng; E,F—100 ng; G,H—200 ng) for 30 minutes. Cells were fixed in 4% buffered paraformaldehyde followed by incubation with PBS containing 0.5% Triton X-100. Fixed cells were stained with Rabbit Anti-γH2AX (pS139) antibody. The antibody was developed using Anti-Rabbit, FITC-conjugated (A,C,E,G). Cells were stained with DAPI (blue) to stain nuclei (B, D, F, H). Results are courtesy of Prof. Y. Shiloh, Department of Human Genetics, Tel-Aviv University, Tel-Aviv, Israel.
Survival curve of cells treated with increasing concentrations of Neocarzinostatin (NCS). U2-OS cells were plated in triplicates and incubated for 48 hr prior to exposure to various NCS doses (Cat. No. N9162). Cell colonies grown for 2 weeks, fixed and stained with 2% crystal violet in 50% ethanol. Colonies were counted under a dissection microscope. Results are courtesy of Prof. Y. Shiloh, Department of Human Genetics, Tel-Aviv University, Tel-Aviv, Israel.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N9162-100UG
| antibiotic activity spectrum | neoplastics |
| assay | ≥90% (SDS-PAGE) |
| concentration | ~0.5 mg/mL |
| form | solution |
| InChI | 1S/C35H35NO12/c1-16-12-19 |
| InChI key | BLXZMHNVKCEIJX-PNKAXBHOSA |
| mode of action | DNA synthesis | interferes |
| Quality Level | 200  |
| shipped in | wet ice |
| SMILES string | N([C@H]1[C@H](O[C@@H]([C@ |
| storage temp. | 2-8°C |
| Application: | Neocarzinostatin from Streptomyces carzinostaticus has been used: • as a DNA damaging agent to treat human fibroblast GM0637 cells • as a radiomimetic antibiotic to induce DNA damage • in combination with Vγ2Vδ2 T cells to determine the cytotoxicity in ovarian cancer cells |
| Biochem/physiol Actions: | Neocarzinostatin is a protein-small molecule complex composed of an enediyne chromophore tightly bound to a 113 amino acid single chain protein. The complex possesses antiproliferative and antitumor activity. The chromophore is the active compound, which is responsible for DNA cleavage; while the apoprotein stabilizes and regulates the availability of the labile chromophore. NCS chromophore is bound non-covalently in a cleft of the binding protein and is dissociable. Upon addition of a thiol, the chromophore forms a highly reactive biradical species that can induce sequence-specific single and double strand breaks in DNA. Neocarzinostatin inhibits DNA synthesis and possesses antitumor activity in various human and animal tumors. NCS inhibits cellular proliferation by inducing G2 cell cycle arrest and apoptosis in both human papillomavirus (HPV) positive and negative cell lines. |
| Biochem/physiol Actions: | Neocarzinostatin is a radiomimetic antibiotic substance. |
| Biochem/physiol Actions: | Protein-small molecule complex composed of an enediyne chromophore tightly bound to a 113-amino acid single chain protein. Chemotherapeutic agent, which inhibits DNA synthesis, possesses antitumor activity, and induces apoptosis. |
| Packaging: | 100 μg in glass bottle |
| Physical form: | Supplied as 100 μg (~9 nmoles) in a solution containing 20 mM MES buffer, pH 5.5. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |