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p-Nitrophenyl Phosphate Liquid Substrate System

SIGMA/N7653 - liquid

Synonym: 4-Nitrophenyl phosphate disodium salt solution; pNPP

CAS Number: 4264-83-9
Empirical Formula (Hill Notation): C6H4NNa2O6P
Molecular Weight: 263.05
MDL Number: MFCD00007319
Linear Formula: O2NC6H4OP(O)(ONa)2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-N7653-100ML 100 mL
 $145.00
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N7653-100ML.
pNPP substrates develop the yellow end-product, p-nitrophenol, when hydrolyzed by alkaline phosphatase. Since this system forms a soluble end product, it is not recommended for blotting or histochemistry.

 

form liquid
InChI 1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
InChI key VIYFPAMJCJLZKD-UHFFFAOYSA-L
Quality Level 200 
shipped in wet ice
SMILES string [Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
storage temp. −20°C
Application: p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Application: Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Biochem/physiol Actions: 4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.
Disclaimer: Stable at least one year when stored below 0 °C.
General description: The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.
Packaging: 100 mL in glass bottle
Physical form: Ready-to-use.
Storage Temp. −20°C
UNSPSC 12352204

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