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4-Nitrophenyl phosphate disodium salt hexahydrate

SIGMA/N4645 - powder, BioReagent, suitable for cell culture, ≥97%

Synonym: p-Nitrophenyl phosphate disodium hexahydrate; Disodium 4-nitrophenyl phosphate hexahydrate; di-Sodium 4-nitrophenyl phosphate; pNPP disodium hexahydrate

CAS Number: 333338-18-4
Empirical Formula (Hill Notation): C6H4NNa2O6P · 6H2O
Molecular Weight: 371.14
EC Number: 224-246-5
MDL Number: MFCD00066288
Linear Formula: O2NC6H4OP(O)(ONa)2 · 6H2O
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-N4645-1G 1 g
 $107.00
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45-N4645-5G 5 g
 $353.00
1/EA		 Add To Favorites
pNPP substrates develop the yellow end-product, p-nitrophenol, when hydrolyzed by alkaline phosphatase. Since this system forms a soluble end product, it is not recommended for blotting or histochemistry.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N4645-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N4645-1VL
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N4645-5G

 

assay ≥97%
biological source synthetic (organic)
form powder
InChI 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI key KAKKHKRHCKCAGH-UHFFFAOYSA-L
product line BioReagent
Quality Level 200 
SMILES string O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
solubility H2O: 100 mg/mL
storage temp. −20°C
technique(s) cell culture | mammalian: suitable
Application: 4-Nitrophenyl phosphate disodium salt hexahydrate has been used:
• as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)
• to treat adherent cells in adhesion assay
• as a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA
Biochem/physiol Actions: p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.
General description: Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
Packaging: 1, 5 g in poly bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥97%
Storage Temp. −20°C
UNSPSC 12352204

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