Neutralization Solution B
SIGMA/N3910
Synonym: DNA neutralization solution; neutralization buffer DNA extraction
Product Type: Chemical
| form | liquid |
| grade | Molecular Biology |
| Quality Level | 200 ![]() |
| storage temp. | −20°C |
| technique(s) | DNA amplification: suitable |
| DNA extraction: suitable |
| Application: | Neutralization Solution B has been used in the modified Sigma Extract-N-Amp™ extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma’s REDExtract-N-Amp™ PCR Reaction Mix. |
| General description: | This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed. |
| Legal Information: | Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | 24 mL in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12161703 |

