Mutanolysin from Streptomyces globisporus ATCCÂ 21553
SIGMA/M9901 - lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified
Synonym: Mutanolysin
CAS Number: 55466-22-3
MDL Number: MFCD00131635
Product Type: Chemical
Gram Positive Bacterial Cell Wall and Gram Negative Bacterial Cell Wall Mutanolysin lyses Listeria and other Gram-positive bacteria.
Structure of Lysing Enzymes - Mutanolysin and Lysozyme
| application(s) | diagnostic assay manufacturing |
| biological source | bacterial ( |
| form | lyophilized powder |
| mol wt | 23 kDa |
| Quality Level | 300  |
| shipped in | wet ice |
| specific activity | ≥4000 units/mg protein (biuret) |
| storage temp. | −20°C |
| suitability | suitable for cell lysis |
| Application: | Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA. |
| Application: | Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA. |
| Biochem/physiol Actions: | Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N |
| Biochem/physiol Actions: | Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N |
| General description: | Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control. |
| Legal Information: | Ficoll is a registered trademark of Cytiva |
| Other Notes: | One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 1000, 5000, 10000, 50000 units in glass bottle |
| Physical form: | Lyophilized powder containing Ficoll® and sodium succinate buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥4000 units/mg protein (biuret) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |