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Methotrexate hydrate

SIGMA/M8407 - powder, BioReagent, suitable for cell culture, ≥98% (HPLC)

Synonym: 4-Amino-10-methylfolic acid hydrate; L-4-Amino-N10-methylpteroylglutamic acid hydrate; L-Amethopterin hydrate; Antifolan hydrate; MTX hydrate; Methylaminopterin hydrate

CAS Number: 133073-73-1
Empirical Formula (Hill Notation): C20H22N8O5 · xH2O
Molecular Weight: 454.44 (anhydrous basis)
MDL Number: MFCD00150847
Linear Formula: C20H22N8O5 · xH2O
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-M8407-100MG 100 mg
 $264.00
1/EA		 Add To Favorites
45-M8407-500MG 500 mg
 $874.00
1/EA		 Add To Favorites

 

antibiotic activity spectrum neoplastics
  parasites
assay ≥98% (HPLC)
form powder
InChI 1S/C20H22N8O5.H2O/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30;/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27);1H2/t13-;/m0./s1
InChI key FPJYMUQSRFJSEW-ZOWNYOTGSA-N
mode of action enzyme | inhibits
product line BioReagent
Quality Level 200 
SMILES string [H]O[H].CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O
solubility H2O: insoluble
storage temp. −20°C
technique(s) cell culture | mammalian: suitable
Application: Methotrexate hydrate has been used:
•  as a dihydrofolate (DHFR) inhibitor in sphere forming assay culture
• as a component of dulbecco′ modified Eagle′s medium (DMEM) for selection of transfected cells
• in viability and co-culture assays

Application: Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems.
Application: Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.
Application: Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.
Biochem/physiol Actions: Methotrexate is a folic acid antagonist.
Biochem/physiol Actions: Methotrexate used to inhibit dihyrofolate reductase and select for DHFR overexpresssion.
Purity ≥98% (HPLC)
Storage Temp. −20°C
UNSPSC 12352207

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