Membrane Scaffold Protein 1E3D1
SIGMA/M7074 - recombinant, expressed in E. coli
Synonym: MSP1E3D1
Product Type: Chemical
The concept of membrane scaffold proteins was originally derived from the naturally occurring apoliporotein A-I component of high density lipoprotein. Phospholipid bilayer nanodiscs have been utilized to incorporate a wide variety of anchored and transmembrane proteins. Nanodiscs can provide improved solubility, homogeneity and more native conditions over traditional microsomal preparation.
The critical component of Nanodiscs is the encircling amphipathic helical protein belt (membrane scaffold protein or MSP). The MSP1D1 construct generates Nanodiscs ~9.8 nm in diameter typically containing ~150 lipid molecules per Nanodisc. The thickness of a Nanodisc can vary depending on the type of phospholipid incorporated (typically 4.6–5.6 nm).
Our two constructs, MSP1D1 and MSP1E3D1, allow the incorporation of a broad range of target protein sizes, generating Nanodiscs of ~10.6 and ~12.9 nm in diameter, respectively.
| ε (extinction coefficient) | 26,600 M-1cm-1 at 280 nm (His-tag-cleaved dissolved in 20 mM Tris pH 7.4, 0.1M NaCl, 0.5mM EDTA and 0.01%NaN3)(lit.) |
| 29,400 M-1cm-1 at 280 nm (uncleaved His-tagged dissolved in 20 mM Tris pH 7.4, 0.1M NaCl, 0.5mM EDTA and 0.01%NaN3)(lit.) | |
| biological source | Streptomyces kanamyceticus |
| description | N-Terminal histidine-tagged |
| form | lyophilized powder |
| mol wt | Mw 32599.98 by amino acid sequence |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| Application: | For an extensive list of citations and protocols visit the Sligar Lab Website at; sligarlab.life.uiuc.edu/n |
| Application: | For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page . |
| Application: | Nanodisc soluble lipid bilayer systems have proven to be a widely applicable means for rendering membrane proteins soluble in aqueous solutions in a native-like bilayer environment where they remain monodisperse and active. The critical component of nanodiscs is the encircling amphipathic helical protein belt (membrane scaffold protein). |
| Application: | The nanodisc system has been employed to incorporate a wide variety of proteins including GPCRs, P450s, bacteriorhodopsin, coagulation factors, cholera toxin, TAR receptor and aromatase. |
| Biochem/physiol Actions: | Generates Nanodiscs ~12.9 nm in diameter |
| General description: | Sequence: GHHHHHHHDYDIPTTENLYFQGSTF |
| General description: | The nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). A soluble nanodisc assembles as the phospholipid forms a bilayer, which is encircled by two amphipathic MSP molecules covering the hydrophobic alkyl chains of the bilayer. The length of the MSP controls the size of the nanodisc structure. MSP1E3D1 yields nanodiscs of ~12.9 nm. The thickness of a nanodisc is dependent on the type of phospholipid incorporated (typically 4.6−5.6 nm). |
| Legal Information: | Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois. • 7,691,414 Membrane scaffold proteins • 7,662,410 Membrane scaffold proteins and embedded membrane proteins • 7,622,437 Tissue factor compositions and methods • 7,592,008 Membrane scaffold proteins • 7,575,763 Membrane scaffold proteins and tethered membrane proteins • 7,083,958 Membrane scaffold proteins • 7,048,949 Membrane scaffold proteins |
| Physical form: | Supplied as a lyophilized histidine-tagged protein with a TEV protease cleavage site stabilized with Tris-HCl, EDTA, and NaCl. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P261 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |