MOPS-EDTA-Sodium Acetate (MESA)
SIGMA/M5755 - powder for RNA electrophoresis buffers
Synonym: MESA Buffer
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M5755-1L
| foreign activity | DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected |
| form | powder |
| grade | Molecular Biology |
| pH | 6.8-7.2(reconstituted per directions) |
| product line | BioReagent |
| Quality Level | 200  |
| solubility | H2O: soluble, faintly yellow |
| storage temp. | room temp |
| technique(s) | electrophoresis: suitable |
| Application: | Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis. |
| General description: | MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution. |
| Packaging: | 1 L in poly bottle |
| Preparation Note: | 1x MESA buffer contains: • 40 mM MOPS • 10 mM sodium acetate • 1 mM EDTA (pH 8.3) |
| Preparation Note: | Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |