Monoclonal Anti-c-Myc antibody produced in mouse
SIGMA/M5546 - clone 9E10, ascites fluid
Synonym: Anti-c-Myc
Product Type: Chemical
Immunofluorescence HEK-293 cells overexpressing N-terminal c-Myc tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:500 Monoclonal Anti-c-Myc Clone: 9E10 (Cat. No. M5546). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence HEK-293 cells overexpressing C-terminal c-Myc tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:500 Monoclonal Anti-c-Myc Clone: 9E10 (Cat. No. M5546). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of human HEK-293T cells overexpressing C-terminal c-Myc tagged fusion protein was separated on SDS-PAGE and probed with Monoclonal Anti-c-Myc Clone: 9E10 (Cat. No. M5546). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:1,000 2. Antibody dilution 1:2,000 3. Antibody dilution 1:4,000
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 9E10, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1:100 using c-myc tagged fusion protein | |
| UniProt accession no. | P01106  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. • Chromatin immunoprecipitation (1 paper) • Enzyme-linked immunosorbent assay (1 paper) • Immunoprecipitation (5 papers) • Western Blotting (5 papers) • Western blotting following immunoprecipitation(1 paper) |
| Application: | Monoclonal anti-c-Myc antibody produced in mouse has been used for Western blotting and immunohistochemistry. Monoclonal Anti-c-Myc antibody has also been used in: • the detection of horseradish peroxidase conjugate in phosphate buffered saline tween-20 (PBST) and 65-kDa mannoprotein (MP65) Dabs • immunoblotting • immunoprecipitation • biochemical and histological studies |
| Biochem/physiol Actions: | c-Myc is a regulator of the cell cycle and aids in the growth of tumors in the liver. Translocations in the gene encoding it have been shown to be linked to Burkitt lymphoma. It has been studied that c-Myc targets many protein-coding genes which enhances the oncogenic properties of c-Myc. |
| Biochem/physiol Actions: | Recognizes both N- terminal and C-terminal c-Myc tagged proteins. |
| Biochem/physiol Actions: | The cellular myelocytomatosis (c-myc) oncogene plays a major role in cellular proliferation, differentiation and apoptosis. It acts as a transcriptional regulator of gene expression. MYC (MYC proto-oncogene) may be essential for cancer initiation, promotion and therapy resistance. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in the poor outcome and invasive treatment, when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | c-Myc is a transcription factor which is over-expressed in a wide range of cancers. |
| General description: | MYC (MYC proto-oncogene) is a transcriptional factor and oncoprotein. c-myc is a member of MYC gene family. It is located on human chromosome 8q24. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor. Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. |
| Immunogen: | synthetic peptide of the human p62c-Myc protein. |
| Physical form: | The product is provided as ascites fluid with 15 mM sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for a maximum of one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |