N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide

SIGMA/M4765 - elastase substrate

Synonym: N-(Methoxysuccinyl)-L-alanyl-L-alanyl-L-prolyl-L-valine 4-nitroanilide; N-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide

CAS Number: 70967-90-7
Empirical Formula (Hill Notation): C₂₇H₃₈N₆O₉
Molecular Weight: 590.63
MDL Number: MFCD00077137
Linear Formula: C₂₇H₃₈N₆O₉
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-M4765-10MG	10 mg	$111.00 1/EA	Add To Favorites
45-M4765-50MG	50 mg	$184.00 1/EA	Add To Favorites
45-M4765-250MG	250 mg	$969.00 1/EA	Add To Favorites
45-M4765-1G	1 g	$3130.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M4765-1G

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98% (HPLC)
form	powder
InChI	1S/C27H38N6O9/c1-15(2)23(26(38)30-18-8-10-19(11-9-18)33(40)41)31-25(37)20-7-6-14-32(20)27(39)17(4)29-24(36)16(3)28-21(34)12-13-22(35)42-5/h8-11,15-17,20,23H,6-7,12-14H2,1-5H3,(H,28,34)(H,29,36)(H,30,38)(H,31,37)/t16-,17-,20-,23-/m0/s1
InChI key	VLVGCNNWNUERRZ-OSAZLGQLSA-N
Quality Level	300
SMILES string	[H][C@]1(CCCN1C(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CCC(=O)OC)C(=O)N[C@@H](C(C)C)C(=O)Nc2ccc(cc2)[N+]([O-])=O
solubility	DMF: soluble 25 mg/mL, clear, colorless to light yellow
storage temp.	−20°C

Application:	MeO-SucAAPVpNA has been used to measure neutrophil elastase activity: • in response to zinc supplementation using human and mice neutrophils in vitro • to determine the functional properties of aged neutrophils to assess their role in supporting metastasis • to determine the anti-elastase action of Sclerocarya birrea (A. Rich.) Hochst
Biochem/physiol Actions:	Human leukocyte elastase (HLE) is a serine proteinase associated with inflammation and tissue degradation. It is one of the most destructive proteases and its role in the development of pulmonary emphysema has been investigated. This peptide is selectively hydrolyzed by HLE, and other elastase of different species and cells. Cleavage of MeO-SucAPVVpNA by an elastase-like protease is critical for the synthesis of platelet-activating factor (PAF) induced by tumor necrosis factor-α (TNF-α) and interleukin-1α (IL-1α). The rate of enzymatic hydrolysis of the substrate is followed by the increase in absorbance due to the release of 4-nitroaniline. The absorbance is routinely measured at 400 nm or higher.
General description:	Substrate for human leukocyte elastase.
Packaging:	1 g in poly bottle
Packaging:	10, 50, 250 mg in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352204

To Order From a Quote