Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse
SIGMA/M4276 - clone MY-32, ascites fluid
Synonym: Anti-Myosin Antibody
MDL Number: MFCD00145920
Product Type: Chemical
Immunofluorescence Staining of formalin-fixed, paraffin-embedded human skeletal muscle sections with Mouse Anti-Myosin (Skeletal, Fast) Clone: MY-32 (Cat. No. M4276) diluted 1:400 using Goat Anti-Mouse IgG, FITC-conjugate.
Immunofluorescence A10 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500 Monoclonal Anti-Myosin (Skeletal, Fast), Clone: MY-32 (Cat. No. M4276). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell and tissue lysates were separated on SDS-PAGE and probed with Mouse Anti-Myosin (Skeletal, Fast) Clone: MY-32 (Cat. No. M4276) diluted to 1:750. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes
1. H9C2; 2. A10; 3. C2C12; 4. Mouse Skeletal Muscle; 5. Rat Skeletal Muscle
1. H9C2; 2. A10; 3. C2C12; 4. Mouse Skeletal Muscle; 5. Rat Skeletal Muscle
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MY-32, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using skeletal muscle tissue |
| indirect immunofluorescence: 1:400 | |
| western blot: 1:1,000 using rabbit leg muscle extract | |
| UniProt accession no. | P12882  |
| Q5SX40 |
| Application: | Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse has been used in: • immunohistochemistry • immunostaining • western blotting at a dilution 1:1000 and 1:90000† • indirect immunofluorescence (dilution 1:400) of formalin-fixed, paraffin-embedded sections of human or animal skeletal muscle tissue preparation. • dot immunobinding on muscle extracts or purified myosin preparations Monoclonal Anti-Skeletal Myosin may be used for staining of human, rabbit, rat, mouse, bovine, chicken and guinea pig skeletal myosin. Monoclonal Anti-Skeletal Myosin antibody to fast-twitch skeletal myosin may be used for detecting cross striated muscle differentiation in tumors. The antibody localizes an epitope on the myosin chain that is stable to the routine formalin-fixation and paraffin-embedding process. |
| Application: | The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000. |
| Biochem/physiol Actions: | Monoclonal Anti-Skeletal Myosin is specific for the myosin heavy chain. It does not stain human or animal cardiac or smooth muscle myosin or cells grown by tissue culture (nonmuscle myosin). It has been demonstrated on human skeletal muscle that the antibody stains the fast twitch (type II) isomyosin molecules. Monoclonal Anti-Skeletal Myosin antibody does react with human rhabdomyosarcomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas. |
| General description: | Monoclonal Anti-Skeletal Myosin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin is a 480,000 dalton protein known to interact with actin in muscle and in non-muscle cells. It contains two identical heavy chains (200,000 daltons each) and four light chains (15,000-26,000 daltons). Myosin molecules consist of two major regions: tails (rods) and heads; they aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Multiple forms of myosin heavy chains exist for each muscle type-skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle. These are designated at type I (slow twitch) and type II (fast-twitch). Type II fibers can be further subdivided in types IIA, IIB, and IIC. |
| Immunogen: | rabbit muscle myosin. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |