Medium 199
SIGMA/M3769 - Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture
Synonym: M199 Medium; TCM 199
Product Type: Chemical
| components | L-glutamine: no phenol red: no HEPES: no sodium pyruvate: no Earle’s salts (5% CO2): yes NaHCO3: no |
| form | powder |
| Quality Level | 500  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Medium 199 has been used: • to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay • to flush the uterine horn of gilts to recover day 12 conceptuses from uteri • to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444) |
| General description: | Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues. |
| Preparation Note: | Formulated to contain 9.4 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 0.1 g/L |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |