2-Mercaptoethanol
SIGMA/M3148 - Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Synonym: β-Mercaptoethanol; 2-
CAS Number: 60-24-2
Empirical Formula (Hill Notation): C2H6OS
Molecular Weight: 78.13
EC Number: 200-464-6
MDL Number: MFCD00004890
Linear Formula: HSCH2CH2OH
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M3148-100ML.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M3148-25ML
| assay | 99% (GC/titration) |
| bp | 157 °C (lit.) |
| concentration | 14.3 M (pure liquid) |
| density | 1.114 g/mL at 25 °C (lit.) |
| expl. lim. | 18 % |
| foreign activity | DNase, RNase, protease, none detected |
| form | liquid |
| grade | Molecular Biology |
| InChI | 1S/C2H6OS/c3-1-2-4/h3-4H, |
| InChI key | DGVVWUTYPXICAM-UHFFFAOYSA |
| pH | 4.5-6 (20 °C, 500 g/L) |
| product line | BioReagent |
| Quality Level | 200  |
| reaction suitability | reagent type: reductant |
| refractive index | n |
| SMILES string | OCCS |
| solubility | H2O: soluble 1 mL/mL |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| electrophoresis: suitable | |
| vapor density | 2.69 (vs air) |
| vapor pressure | 1 mmHg ( 20 °C) |
| Application: | BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. |
| Application: | Reducing agent β-mercaptoethanol or 2-mercaptoethanol has been used: • as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line) • to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells • to trypsinize mouse embryonic stem cells |
| Packaging: | 25, 100, 250, 500 mL in glass bottle |
| Symbol |     GHS05,GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H301 + H331 - H310 - H315 - H317 - H318 - H361d - H373 - H410 |
| Precautionary statements | P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338 |
| Hazard Codes | T,N |
| Risk Statements | 23/24/25-38-41-43-48/22-50/53 |
| Safety Statements | 26-36/37/39-45-60-61 |
| RIDADR | UN 2966 6.1 / PGII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 154.4 °F - closed cup |
| Flash Point(C) | 68 °C - closed cup |
| Purity | 99% (GC/titration) |
| bp | 157 °C (lit.) |
| Density | 1.114 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161504 |