Thiazolyl Blue Tetrazolium Bromide

SIGMA/M2128 - 98%

Synonym: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; MTT; Methylthiazolyldiphenyl-tetrazolium bromide

CAS Number: 298-93-1
Empirical Formula (Hill Notation): C₁₈H₁₆BrN₅S
Molecular Weight: 414.32
EC Number: 206-069-5
MDL Number: MFCD00011964
Linear Formula: C₁₈H₁₆BrN₅S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-M2128-100MG	100 mg	$36.30 1/EA	Add To Favorites
45-M2128-250MG	250 mg	$57.30 1/EA	Add To Favorites
45-M2128-500MG	500 mg	$101.00 1/EA	Add To Favorites
45-M2128-1G	1 g	$177.00 1/EA	Add To Favorites
45-M2128-5G	5 g	$659.00 1/EA	Add To Favorites
45-M2128-10G	10 g	$1160.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M2128-100MG.

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing hematology histology
assay	98%
color	dark yellow
form	powder
InChI	1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
InChI key	AZKSAVLVSZKNRD-UHFFFAOYSA-M
mp	195 °C (dec.) (lit.)
Quality Level	200
SMILES string	[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
solubility	2-methoxyethanol: soluble 20 mg/mL
	ethanol: soluble 20 mg/mL
	H₂O: 5 mg/mL
storage temp.	2-8°C
technique(s)	titration: suitable

Application:	Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
Application:	Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Application:	Thiazolyl Blue Tetrazolium Bromide has been used for the following assays: • Proliferation assay. • TNF (tumor necrosis factor) bioassay.
Packaging:	1, 5, 10 g in glass bottle
Packaging:	100, 250 mg in glass insert
Packaging:	500 mg in glass bottle

Symbol	GHS07,GHS08
Signal word	Warning
Hazard statements	H315 - H319 - H335 - H341
Precautionary statements	P201 - P202 - P261 - P302 + P352 - P305 + P351 + P338 - P308 + P313
Hazard Codes	Xn
Risk Statements	36/37/38-68
Safety Statements	26-36/37
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	98%
mp	195 °C (dec.) (lit.)
Storage Temp.	2-8°C
UNSPSC	12352111

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