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MTT Formazan

SIGMA/M2003 - powder

Synonym: 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan; Thiazolyl blue formazan

CAS Number: 57360-69-7
Empirical Formula (Hill Notation): C18H17N5S
Molecular Weight: 335.43
MDL Number: MFCD00080731
Linear Formula: C18H17N5S
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-M2003-1G 1 g
 $171.00
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M2003-1G

 

application(s) diagnostic assay manufacturing
hematology
histology
assay ≥97% (TLC)
color purple
form powder
InChI 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
InChI key GIFKVQOPAJDJLO-SZVLODPRSA-N
Quality Level 200 
SMILES string Cc1nc(N=NC(=NNc2ccccc2)c3ccccc3)sc1C
solubility acetone: 1 mg/mL
storage temp. room temp
Application: MTT Formazan has been used to measure cell viability.
Biochem/physiol Actions: MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.
Packaging: 1 g in glass bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥97% (TLC)
Storage Temp. room temp
UNSPSC 12171500

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