Anti-MAP2 (2a+2b) antibody, Mouse monoclonal

SIGMA/M1406 - clone AP-20, ascites fluid

Synonym: Anti-Microtubule Associated Protein 2

MDL Number: MFCD00164684
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-M1406-100UL	100 µL	$705.00 1/EA	Add To Favorites
45-M1406-.2ML	0.2 mL	$871.00 1/EA	Add To Favorites

Immunohistochemistry-immunofluorescence JOURNAL CITATION: Rod microglia: elongation, alignment, and coupling to form trains across the somatosensory cortex after experimental diffuse brain injury. By: Ziebell, J. M., Taylor, S. E., et al. in J Neuroinflammation, 2012. PubMed ID: 23111107 Image collected and cropped by CiteAb from the following publication, (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-9-247), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.

Immunocytochemistry-immunoflourescence JOURNAL CITATION: HINT1 protein cooperates with cannabinoid 1 receptor to negatively regulate glutamate NMDA receptor activity. By: Vicente-Sánchez, A., Sánchez-Blázquez, P., et al. in Mol Brain, 2013. PubMed ID: 24093505 Image collected and cropped by CiteAb from the following publication, (http://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-6-42), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.

Immunoblotting
Rat brain cortex whole cell extracts were separated on SDS-PAGE, blotted with Monoclonal Anti-MAP2 (2A+2B) (Cat. No. M1406) and developed using Goat Anti-Mouse IgG, Alkaline Phosphatase (Cat. No. A1293) and NBT/BCIP substrate. (Lane A) Antibody dilution 1:500; (Lane B) Negative control (only secondary antibody).

Immunofluorescence SH-SY-5Y cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500 Monoclonal Anti-MAP2 (2a+2b) Clone: AP-20 (Cat. No. M1406). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence B35 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:100 Monoclonal Anti-MAP2 (2a+2b) Clone: AP-20 (Cat. No. M1406). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Properties
Descriptions
Safety Info.
Basic Data

antibody form	ascites fluid
antibody product type	primary antibodies
biological source	mouse
clone	AP-20, monoclonal
conjugate	unconjugated
contains	15 mM sodium azide
isotype	IgG1
mol wt	apparent mol wt 280 kDa
Quality Level	200
shipped in	dry ice
species reactivity	Xenopus, mouse, quail, human, bovine, rat, aquatic salamander
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	immunocytochemistry: suitable
	western blot: 1:500 using rat brain enriched microtubule protein preparation or rat cerebral cortex extract
UniProt accession no.	P11137

Application:	Monoclonal Anti-MAP2 (2a+2b) antibody has been used in immunohistochemistry, immunocytochemistry and antibody characterization.
Biochem/physiol Actions:	Microtubule-associated protein 2 (MAP2) interacts with microtubules to maintain the structure of dendrites. It plays a major role in supporting the actin cytoskeleton in spines, binding and nucleating filamentous actin (f-actin) to modulate spine morphology.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	Microtubule-associated protein 2 (MAP2) is a neuronal protein. It belongs to the MAP2/Tau family. This gene is located to human chromosome 2q34-q35. MAP2 is present in neurons. There are three forms of MAP2; two are similarly sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton. Monoclonal Anti-MAP2 (2a + 2b) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Immunogen:	bovine MAP2
Other Notes:	2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Physical form:	The product is provided as ascites fluid with 15 mM sodium azide as a preservative.
Preparation Note:	For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Hazard Codes	Xn
Risk Statements	21/22
Safety Statements	36/37
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1

Storage Temp.	−20°C
UNSPSC	12352203

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