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Calcein Blue

SIGMA/M1255 - fluorescent dye

Synonym: 4-Methylumbelliferone-8-methyliminodiacetic acid

CAS Number: 54375-47-2
Empirical Formula (Hill Notation): C15H15NO7
Molecular Weight: 321.28
EC Number: 259-129-8
MDL Number: MFCD00006867
Linear Formula: C15H15NO7
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-M1255-1G 1 g
 $81.20
1/EA		 Add To Favorites
45-M1255-10G 10 g
 $466.00
1/EA		 Add To Favorites
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M1255-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M1255-1G

 

application(s) diagnostic assay manufacturing
hematology
histology
color white to light yellow
fluorescence λex 322 nm; λem 445 nm in methanol
form powder
InChI 1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
InChI key RJPSHDMGSVVHFA-UHFFFAOYSA-N
Quality Level 200 
SMILES string CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
solubility 1 M NaOH: 50 mg/mL
storage temp. room temp
suitability suitable for indicator for EDTA titration
technique(s) titration: suitable
Application: Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.
General description: Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.
Packaging: 1, 10 g in poly bottle
Storage Temp. room temp
UNSPSC 12171500

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