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Minimum Essential Medium Eagle

SIGMA/M0275 - 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym: EMEM; MEM; MEM

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-M0275-100ML 100 mL
$36.70
1/EA
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45-M0275-500ML 500 mL
$74.30
1/EA
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45-M0275-6X500ML 500 mL
$406.00
6/EA
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components phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no
concentration 10 ×
form liquid
impurities endotoxin, tested
Quality Level 400 
shipped in ambient
sterility sterile-filtered
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
Application: Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.
Application: Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel). It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.
Preparation Note: Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352207

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