Anti-LC3 antibody produced in rabbit
SIGMA/L8918 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Lc3; LC3 Antibody - Anti-LC3 antibody produced in rabbit; Lc3 Antibody; Anti-MAP1 light chain 3-like protein 1; Anti-MAP1A/1B light chain 3A; Anti-MAP1A/1BLC3; Anti-MAP1A/MAP1B LC3A; Anti-MAP1ALC3; Anti-MAP1BLC3; Anti-MAP1LC3A; Anti-MAP1LC3B; Anti-
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: MCOLN1 is a ROS sensor in lysosomes that regulates autophagy. By: Zhang, X., Cheng, X., et al. in Nat Commun, 2016. PubMed ID: 27357649 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms12109), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: QSOX1 inhibits autophagic flux in breast cancer cells. By: Poillet, L., Pernodet, N., et al. in PLoS One, 2014. PubMed ID: 24475161 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0086641), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: QSOX1 inhibits autophagic flux in breast cancer cells. By: Poillet, L., Pernodet, N., et al. in PLoS One, 2014. PubMed ID: 24475161 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0086641), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry (Paraffin) Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. L8918, Anti-LC3 antibody. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons & neuropil of human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Formalin Fixed Paraffin Embedded (FFPE) rat cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. L8918, Anti-LC3 antibody. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was also observed in granular & molecular layers, as well as Purkinje cells of rat cerebellum tissue sections.
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 2 μg/mL Rabbit Anti-LC3 (Cat. No. L8918). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Rat brain whole extract was separated by SDS-PAGE, blotted with Rabbit Anti-LC3 (Cat. No. L8918) and developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1: Antibody 0.5 μg/mL 2: Antibody 1 μg/mL 3: Antibody 1 μg/mL + LC3 immunizing peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 16-18 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): 5-10 μg using extracts of human U87 cells | |
| western blot: 1-2 μg/mL using whole extracts of rat and mouse brain | |
| UniProt accession no. | Q9H492  |
| Application: | Anti-LC3 antibody produced in rabbit has been used in: • western blotting • immunofluorescence • immunohistochemistry • filter retardation assay • immunocytochemistry |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (3 papers) Immunohistochemistry (1 paper) Western Blotting (21 paper) |
| Biochem/physiol Actions: | The gene MAP1LC3A (microtubule associated protein 1 light chain 3 α) encodes a protein that serves as a marker of autophagosomes. It is found to be expressed on surviving oligodendrocytes in Nasu-Hakola disease brains and may be involved in the induction of oligodendrogliopathy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene LC3, also referred to as MAP1LC3A (microtubule associated protein 1 light chain 3 α), is a homologue of Apg8p necessary for autophagy in yeast. The cytosolic form upon processing gets localized to the autophagosome membranes. The gene is mapped to human chromosome 20q11.22. LC3 exists in cells as cytoplasmic LC3-I (18kDa) and LC3-II (16kDa). |
| Immunogen: | Synthetic peptide corresponding to amino acids of human LC3A isoform a, conjugated to KLH via C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |