Anti-LC3A antibody produced in rabbit
SIGMA/L8793 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-MAP1 light chain 3-like protein 1; Anti-MAP1A/1B light chain 3 A; Anti-MAP1A/MAP1B LC3 A; Anti-MAP1ALC3; Anti-MAP1BLC3; Anti-MAP1LC3A; Anti-
Product Type: Chemical
Immunoblotting Rat brain whole extract was separated by SDS-PAGE, blotted with Rabbit Anti-LC3A (Cat. No. L8793) and developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1: Antibody 0.5 μg/mL 2: Antibody 1 μg/mL 3: Antibody 1 μg/mL + LC3A immunizing peptide
Western Blotting Whole extract of Mouse brain were separated on SDS-PAGE and probed with Anti-LC3A antibody produced in Rabbit (Cat. No. L8793). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 16-18 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using extracts of human U87 cells |
| western blot: 0.5-1 μg/mL using whole extracts of rat and mouse brain | |
| UniProt accession no. | Q9H492  |
| Application: | Anti-LC3A antibody produced in rabbit has been used in • immunohistochemistry • immunostaining • western blotting |
| Biochem/physiol Actions: | Anti-LC3A antibody recognizes human and rat microtubule-associated protein light chain 3-A (LC3A-I) and LC3A-II by immunoblotting and immunoprecipitation (~18 kDa and ~16 kDa, respectively) and mouse LC3A-I (~18 kDa). |
| Biochem/physiol Actions: | Microtubule-associated protein light chain 3-I (LC3-I) is modified to a membrane-bound form, LC3-II (a LC3-phospholipid conjugate), by mammalian autophagy related 7 (Atg7) and Atg3, which are E1- and E2-like enzymes, respectively. The amount of LC3 II correlates with the extent of autophagosome formation. LC3A can also be as an autophagosomal marker. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Microtubule-associated proteins 1A/1B light chain 3 or MAP1LC3 is a cellular protein that is involved in the formation of autophagosomes . MAP1LC3 genes have three known human isoforms, namely, MAP1LC3A, MAP1LC3B and MAP1LC3C. These isoforms have different responses to amino acid starvation and varied sub-cellular localization patterns . Anti-LC3A antibody reacts with human and rat LC3A-I (approx. 18 kDa) and LC3A-II (approx. 16 kDa) by immunoblotting and immunoprecipitation. The antibody is also specific for mouse LC3A-I (approx. 18 kDa). Specific cell treatment and/or long exposure may be required for the detection of mouse LC3A-II. The immunizing peptide specifically inhibits the detection of the LC3A bands by immunoblotting. |
| Immunogen: | Synthetic peptide corresponding to amino acids of human LC3A isoform α, conjugated to KLH via C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |