Lysozyme from human neutrophils

SIGMA/L8402 - ≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)

Synonym: Mucopeptide N-acetylmuramoylhydrolase; Muramidase

CAS Number: 9001-63-2
MDL Number: MFCD00131557
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-L8402-.1MG	0.1 mg	$189.00 1/EA	Add To Favorites

Lysozyme specificity: Peptidoglycans are polymers of β-(1-4)-N-Acetyl-D-glucosamine units. Alternating residues are modified to form N-acetylmuramic acid with the addition of lactate to form branching links to the tetrapeptide.

Properties
Descriptions
Safety Info.
Basic Data

application(s)	cell analysis
assay	≥95% (SDS-PAGE)
biological source	human neutrophils
form	lyophilized powder
InChI	1S/C99H159N37O23/c1-11-50(8)77(132-72(139)46-120-81(145)63(32-33-70(101)137)124-88(152)69-31-21-39-136(69)93(157)68(43-73(140)141)131-84(148)62(29-19-37-116-98(109)110)125-90(154)75(48(4)5)135-91(155)76(49(6)7)133-80(144)57(100)24-16-34-113-95(103)104)92(156)126-60(27-17-35-114-96(105)106)82(146)121-51(9)78(142)129-66(41-54-45-119-59-26-15-13-23-56(54)59)87(151)134-74(47(2)3)89(153)122-52(10)79(143)128-65(40-53-44-118-58-25-14-12-22-55(53)58)85(149)123-61(28-18-36-115-97(107)108)83(147)130-67(42-71(102)138)86(150)127-64(94(158)159)30-20-38-117-99(111)112/h12-15,22-23,25-26,44-45,47-52,57,60-69,74-77,118-119H,11,16-21,24,27-43,46,100H2,1-10H3,(H2,101,137)(H2,102,138)(H,120,145)(H,121,146)(H,122,153)(H,123,149)(H,124,152)(H,125,154)(H,126,156)(H,127,150)(H,128,143)(H,129,142)(H,130,147)(H,131,148)(H,132,139)(H,133,144)(H,134,151)(H,135,155)(H,140,141)(H,158,159)(H4,103,104,113)(H4,105,106,114)(H4,107,108,115)(H4,109,110,116)(H4,111,112,117)/t50-,51-,52-,57-,60-,61-,62-,63-,64-,65-,66-,67-,68-,69-,74-,75-,76-,77-/m0/s1
InChI key	ZJCXKXFAOCLSRV-RYLVIXIQSA-N
Quality Level	200
recombinant	expressed in E. coli
SMILES string	N1([C@@H](CCC1)C(=O)N[C@@H](CCC(=O)N)C(=O)NCC(=O)N[C@@H]([C@H](CC)C)C(=O)N[C@@H](CCCNC(=N[H])N)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc4c5c([nH]c4)cccc5)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc2c3c([nH]c2)cccc3)C(=O)N[C@@H](CCCNC(=N[H])N)C(=O)N[C@@H]
specific activity	≥100,000 units/mg protein (E1%/280)
storage temp.	−20°C
suitability	suitable for cell lysis
technique(s)	cell based assay: suitable
UniProt accession no.	P61626

Application:	Lysozyme from human neutrophils has been used in the checkerboard method for establishing a synergy between peptidomimetic 1 and immune effector molecules against E. coli.
Biochem/physiol Actions:	Lysozymes participates in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.
General description:	Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.
Other Notes:	One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
Other Notes:	View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Physical form:	Lyophilized from 50 mM sodium acetate, pH 6.0, with 100 mM NaCl

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥95% (SDS-PAGE)
activity	specific activity: ≥100,000 units/mg protein (E1%/280)
Storage Temp.	−20°C
Enzyme Commission (EC) Number	3.2.1.17 ( BRENDA \| IUBMB )
UNSPSC	12352204

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