Lectin from Galanthus nivalis (snowdrop)
SIGMA/L8275 - lyophilized powder
Synonym: Galanthus nivalis lectin; GNL
MDL Number: MFCD00163718
Product Type: Chemical
Agglutination
assay: Lectin from Galanthus nivalis (snowdrop) (Cat. No.
L8275) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top
to bottom of plate) and incubated with rabbit erythrocytes (4% v/v RBC) to calculate
Minimum agglutination titer (MAT). Agglutination is defined as the inability
of cells to form a tight button. PBS was used as blank.
assay: Lectin from Galanthus nivalis (snowdrop) (Cat. No.
L8275) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top
to bottom of plate) and incubated with rabbit erythrocytes (4% v/v RBC) to calculate
Minimum agglutination titer (MAT). Agglutination is defined as the inability
of cells to form a tight button. PBS was used as blank.
SDS-PAGE Lectin from Galanthus nivalis (snowdrop) (Cat. No. L8275) was assessed on SDS-PAGE.
| composition | Protein, ≥90% Lowry |
| form | lyophilized powder |
| impurities | salt, essentially free |
| potency | <20 μg per mL agglutination activity |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Analysis Note: | Agglutination activity is expressed in μg/ml and is determined from serial dilution in phosphate buffered saline, pH 7.2 of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of rabbit erythrocytes after 1 hr incubation at 25 °C. |
| Application: | Lectin from Galanthus nivalis (snowdrop) has been used: • to coat ELISA plates • as reverse transcriptase inhibitor • in ELISA for detection of envelope glycoproteins of the human and simian immunodeficiency viruses |
| Biochem/physiol Actions: | GNL agglutinates rabbit erythrocytes; human erythrocytes are not agglutinated. GNL sugar specificity is directed to the nonreducing end of the terminal α- |
| Biochem/physiol Actions: | Lectin from Galanthus nivalis (snowdrop) provide resistance against pests and are used in transgenic plants. Snowdrop lectin functions as a carrier to transport fused proteins to the hemolymph as it crosses gut epithelium of target species to exhibit insecticidal effects. |
| General description: | Lectin from Galanthus nivalis (snowdrop) is a tetrameric, 12 kDa mannose binding lectin. It belongs to the family, Amaryllidacea. It is specific to α-1,3- or 1,6- linked |
| Packaging: | 5 mg in glass bottle |
| Preparation Note: | Purified by affinity chromatography according to Kaku. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |