Anti-Laminin antibody, Mouse monoclonal
SIGMA/L8271 - clone LAM-89, purified from hybridoma cell culture
Synonym: Anti-Laminin Antibody
MDL Number: MFCD00162586
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded human tongue section, enzyme-treated was stained with 1:2,000 Monoclonal Anti-Laminin Clone: LAM-89 (Cat. No. L8271). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunohistochemistry Formalin-fixed, paraffin-embedded human placenta section, enzyme-treated was stained with 1:2,000 Monoclonal Anti-Laminin Clone: LAM-89 (Cat. No. L8271). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LAM-89, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | rabbit, lizard, sheep, carp, canine, chicken, rat, guinea pig, goat, frog, snake |
| species reactivity | feline, human, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using enzyme-treated human tissue sections |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P07942  |
| P25391 |
| Application: | Monoclonal Anti-laminin antibody can be used in indirect immunostaining to determine the distribution of laminin. It may also be used for ELISA, Immunofluorescence and western blotting. |
| Application: | Monoclonal Anti-Laminin antibody: • immunohistochemistry to stain matrix proteins • double immunofluorescence staining • indirect immunofluorescence • immunogold labelling for electron microscopy |
| Biochem/physiol Actions: | Laminin is a cell substrate adhesion protein and thus has a crucial role in promoting neurite regeneration and modulating cellular functions like differentiation, cell shape and cell movement because it appears to be an important cell substrate adhesion protein. It plays an important role in many aspects of the cell biology. Variations in the expression of this protein have been observed in embryogenesis, organogenesis, post traumatic healing and cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Laminin, a 900kD (approx.) complex extracellular glycoprotein, is the most abundant structural and biologically active component present in the basement membranes. It is a cell substrate adhesion protein and thus has a crucial role in promoting neurite regeneration and modulating cellular functions like differentiation, cell shape and cell movement. Monoclonal Anti-laminin antibody can be used in immunohistochemistry to classify various disease processes involving basement membranes, to mark blood vessel walls of different species and to determine the origin and classification of human tumours. It can also be used in dot blot assays. Mouse anti- laminin antibody reacts specifically with purified human laminin but not with collagen IV, fibronectin, vitronectin or chondroitin sulfate types A, B, and C. |
| General description: | Monoclonal Anti-Laminin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with an approximate molecular weight of 900 kDa. Laminin is composed of one A chain (400 kDa) one B1 chain (215 kDa) and one B2 chain (205 kDa) all held together by disulfide bonds. Laminin is only found in significant quantities in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. |
| Immunogen: | human laminin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |