Lectin from Ulex europaeus (gorse, furze)
SIGMA/L8262 - biotin conjugate, lyophilized powder
Synonym: Ulex europaeus agglutinin; UEA-I
Product Type: Chemical
Agglutination
assay: Lectin from Ulex europaeus (gorse, furze) (Cat.
No. L8262) at a concentration of ~1mg solid/mL was serially diluted (2-fold,
top to bottom of plate) and incubated with human erythrocytes type: O (4% v/v
RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined
as the inability of cells to form a tight button. PBS was used as blank.
assay: Lectin from Ulex europaeus (gorse, furze) (Cat.
No. L8262) at a concentration of ~1mg solid/mL was serially diluted (2-fold,
top to bottom of plate) and incubated with human erythrocytes type: O (4% v/v
RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined
as the inability of cells to form a tight button. PBS was used as blank.
| composition | Protein, ~85% E |
| conjugate | biotin conjugate |
| extent of labeling | ~3 mol biotin per mol protein |
| form | lyophilized powder |
| potency | <50 μg/mL agglutination activity |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Analysis Note: | Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C. |
| Application: | Lectin from Ulex europaeus (gorse, furze) has been used: • as a Paneth cells marker in immunohistochemistry at 1:1,000 dilution • in double staining for endothelial cells for quantitation of blood vessels • in histochemical staining of bronchial epithelial cells |
| Biochem/physiol Actions: | Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for |
| Other Notes: | Conjugates are prepared from affinity purified lectin. |
| Packaging: | 0.5, 2 mg in serum bottle |
| Packaging: | Package size based on protein content. |
| Physical form: | Contains sodium citrate |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |