Lectin from Wisteria floribunda
SIGMA/L8258 - lyophilized powder
Synonym: Wisteria floribunda agglutinin; WFA
MDL Number: MFCD00166187
Product Type: Chemical
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| composition | Protein, ~95% E |
| form | lyophilized powder |
| potency | <16 μg/mL agglutination activity |
| Quality Level | 200  |
| storage temp. | −20°C |
| Analysis Note: | Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C. |
| Application: | Lectin from Wisteria floribunda has been used: • to study the perineuronal nets and parvalbumin nets in mouse brain by immunofluorescence • to evaluate the amount of N-acetylgalactosamine (GalNAc) transferred to Gaussia luciferase (GLuc) substrates by microplate assay • to study its binding effects on perineuronal nets |
| Biochem/physiol Actions: | Lectin binds with high affinity to the glycans of polysaccharides, glycolipids, and glycoproteins. It is involved in plant defense. |
| Biochem/physiol Actions: | WFA is not blood group specific, but has an affinity for N-acetyl- |
| General description: | Lectin is a carbohydrate-binding protein found in plant roots, rhizomes, leaves, seeds, and stems. Lectin is located in the cytoplasm as well as in the nucleus. |
| Packaging: | 1, 5 mg in glass bottle |
| Packaging: | Package size based on protein content |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |