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Lectin from Arachis hypogaea (peanut)

SIGMA/L7759 - peroxidase conjugate, lyophilized powder

Synonym: PNA; Peanut agglutinin

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-L7759-250UG 250 µg
 $147.00
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45-L7759-1MG 1 mg
 $483.00
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Lanes 1. Urokinase from human urine (Sigma U0633) (glcNAc,Fuc) 2. Factor IX Human (Sigma F0806) 3. Complement component C1q from human serum (Sigma C1740) (galNAc, Gal) 4. a1-Antitrypsin from human plasma (Sigma A3106) 5. Fetuin (Sigma F2379) 6. Invertase glycoprotein Standard (Sigma I0408) 7. IgG1 Monoclonal Anti-Flag M2 Ab produced in mouse (Sigma F3165) 8. IgG2a Monoclonal Anti-AMY1A Ab produced in mouse (Sigma WH0000276M4) 9. Recombinant Human t-Plasminogen Activator/tPA (R&D Systems 7449-SE-010) 10. Epidermal growth factor receptor human (Sigma E3641) (glcNAc) 11. Apolipoprotein C-III from human plasma (Sigma A3106) 12. Thrombospondin from human platelets (Calbiochem 605225)

 

biological source Arachis hypogaea (peanut)
conjugate peroxidase conjugate
form lyophilized powder
peroxidase activity ≥20 units/mg protein
Quality Level 200 
storage temp. −20°C
technique(s) western blot: suitable
Application: General Western Blot Protocol:
• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
    • Run 4-20% Bis-Tris SDS page gel
    • Transfer gel to a PVDF membrane
    • Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
    • Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
    • Wash membrane 5 x 5 minutes with 25ml RIPA buffer
    • Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions: PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.
Other Notes: One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Packaging: 250 μg in serum bottle
Physical form: Contains sodium citrate
Preparation Note: Prepared from peroxidase (P8375) using a modification of a published method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
activity peroxidase activity: ≥20 units/mg protein
Storage Temp. −20°C
UNSPSC 12352202

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