Anti-LC3B antibody produced in rabbit
SIGMA/L7543 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: LC3B Antibody - Anti-LC3B antibody produced in rabbit; Lc3 Antibody Sigma; Lc3B Antibody; Anti-
MDL Number: MFCD09265362
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: The interplays between autophagy and apoptosis induced by enterovirus 71. By: Xi, X., Zhang, X., et al. in PLoS One, 2013. PubMed ID: 23437282 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0056966), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: The interplays between autophagy and apoptosis induced by enterovirus 71. By: Xi, X., Zhang, X., et al. in PLoS One, 2013. PubMed ID: 23437282 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0056966), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Neuregulin promotes incomplete autophagy of prostate cancer cells that is independent of mTOR pathway inhibition. By: Schmukler, E., Shai, B., et al. in PLoS One, 2012. PubMed ID: 22606295 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0036828), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Functional Assay HeLa cells were incubated in EBSS medium for 2 hr in the presence of 100 nM Bafilomycin A1 for autophagosomes accumulation. Autophagosome staining is demonstrated in the experimentally induced HeLa cells (A) while no autophagosome staining is demonstrated in the untreated cells (B). Cells were fixed and permeabilized with cold methanol followed by cold acetone and stained with 5 μg/mL Rabbit Anti-LC3B (Cat. No. L7543), a marker for autophagosome membranes. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Results Autophagosome staining is demonstrated in the experimentally induced HeLa cell (A). No autophagosome staining is demonstrated in the untreated cell (B).
Immunofluorescence
HeLa cells were incubated in EBSS medium for 2 h in the presence of 100 nM Bafilomycin A1 for autophagosomes accumulation. Cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 5 μg/mL Rabbit Anti-LC3B (Cat. No. L7543). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
HeLa cells were incubated in EBSS medium for 2 h in the presence of 100 nM Bafilomycin A1 for autophagosomes accumulation. Cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 5 μg/mL Rabbit Anti-LC3B (Cat. No. L7543). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoblotting
Whole extracts of human HEK-293T cells (Lane A) or HEK-293T cells pre-treated with tunicamycin for 48 h (Lane B) were separated on SDS-PAGE and blotted.
Upper panel: Anti-Actin (Cat. No. A3853). Lower panel: 0.5 μg/mL Rabbit Anti-LC3B (Cat. No. L7543). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate.
Whole extracts of human HEK-293T cells (Lane A) or HEK-293T cells pre-treated with tunicamycin for 48 h (Lane B) were separated on SDS-PAGE and blotted.
Upper panel: Anti-Actin (Cat. No. A3853). Lower panel: 0.5 μg/mL Rabbit Anti-LC3B (Cat. No. L7543). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | functional assay Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 16-18 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using human HeLa cells |
| western blot: 1-2 μg/mL using whole extracts of rat and mouse brain | |
| UniProt accession no. | Q9GZQ8  |
| Application: | Anti-LC3B antibody produced in rabbit has been used in western blotting and immunohistochemistry. |
| Application: | Anti-LC3B antibody produced in rabbit has been used in western blotting and immunohistochemistry. |
| Biochem/physiol Actions: | By immunoblotting, Anti-LC3B antibody recognizes human, rat, and mouse LC3B-I and LC3B-II (~18 kDa and ~16 kDa, respectively). |
| Biochem/physiol Actions: | MAP1LC3B-I (microtubule associated protein 1 light chain 3 β-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker. |
| Biochem/physiol Actions: | Microtubule-associated protein 1 light chain 3 (MAPLC3/LC3) expression level correlates with the extent of autophagosome formation. LC3B can be used as an autophagosomal marker. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Microtubule-associated protein 1 light chain 3 (MAPLC3/LC3) was first identified in rat as a protein that co-purifies with microtubule-associated protein 1A and 1B from rat brain. LC3 exists in cells in two forms. One is cytoplasmic, LC3-I (18 kDa) and the other, LC3-II (16 kDa) is associated with the autophagosome membrane. LC3-I is modified to a membrane-bound form, LC3-II (a LC3-phospholipid conjugate), by mammalian autophagy related protein 7 (Atg7) and Atg3, which are E1- and E2-like enzymes, respectively. Three human orthologs of the rat LC3, named MAP1LC3A/LC3A, MAP1LC3B/LC3B, and MAP1LC3C/ LC3C, were identified. The human proteins exhibit two forms representing the cytosolic (type I) and the membrane associated (type II) forms. The three human isoforms show different expression patterns in human tissues. |
| General description: | The gene MAP1LC3B (microtubule associated protein 1 light chain 3 β) is a homolog of ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I. |
| Immunogen: | synthetic peptide corresponding to amino acids 2-15 of human LC3B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence differs by one amino acid in rat and mouse. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |